Human HP CagA lgA ELISA Kit

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  • Catalog
    E018112
  • species
    Human
  • GeneHP CagA lgA
  • Standard CurveHuman HP CagA lgA ELISA Kit
  • Other Species Mouse HP-CagA-lgA ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman HP CagA lgA ELISA Kit allows for the in vitro quantitative determination of HP CagA lgA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyMicrobiology
  • Product Description
    specifical
    Principle of the Assay: HP-CagA-IgG ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with HP-CagA antigen. Standards or samples are then added to the microtiter plate wells and HP-CagA-IgG if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of HP-CagA-IgG present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated HP-CagA antigen is added to each well to "sandwich" the HP-CagA-IgG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HP-CagA-IgG and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HP-CagA-IgG concentration in each sample is interpolated from this standard curve.


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