Human HHIP ELISA Kit

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  • Alternative name

    Human HHIP ELISA Kit;Human HIP ELISA Kit;Human hedgehog interacting protein ELISA Kit;Human hedgehog-interacting protein ELISA Kit;

  • Catalog
    E018097
  • species
    Human
  • GeneHHIP
  • Standard CurveHuman HHIP ELISA Kit
  • Other Species Mouse Hhip ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Hedgehog Interacting Protein (HHIP). No significant cross-reactivity or interference between Hedgehog Interacting Protein (HHIP) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.107ng/mL.
  • Intended UseHuman HHIP ELISA Kit allows for the in vitro quantitative determination of HHIP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hedgehog Interacting Protein (HHIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hedgehog Interacting Protein (HHIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hedgehog Interacting Protein (HHIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hedgehog Interacting Protein (HHIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Hedgehog-interacting protein Protein information
  • Uniprot ID HHIP_HUMAN
  • Uniprot AC Q96QV1; Q6PK09; Q8NCI7; Q9BXK3; Q9H1J4; Q9H7E7;
  • UniGene Hs.507991;
  • GeneID 64399
  • KEGG hsa:64399;
  • Human Hedgehog-interacting protein Protein SEQUENCE
  • SEQUENCE 700 AA; 78851 MW; CC1CB3435E29303A CRC64;

    MLKMLSFKLL LLAVALGFFE GDAKFGERNE GSGARRRRCL NGNPPKRLKR

    RDRRMMSQLE LLSGGEMLCG GFYPRLSCCL RSDSPGLGRL ENKIFSVTNN

    TECGKLLEEI KCALCSPHSQ SLFHSPEREV LERDLVLPLL CKDYCKEFFY

    TCRGHIPGFL QTTADEFCFY YARKDGGLCF PDFPRKQVRG PASNYLDQME

    EYDKVEEISR KHKHNCFCIQ EVVSGLRQPV GALHSGDGSQ RLFILEKEGY

    VKILTPEGEI FKEPYLDIHK LVQSGIKGGD ERGLLSLAFH PNYKKNGKLY

    VSYTTNQERW AIGPHDHILR VVEYTVSRKN PHQVDLRTAR VFLEVAELHR

    KHLGGQLLFG PDGFLYIILG DGMITLDDME EMDGLSDFTG SVLRLDVDTD

    MCNVPYSIPR SNPHFNSTNQ PPEVFAHGLH DPGRCAVDRH PTDININLTI

    LCSDSNGKNR SSARILQIIK GKDYESEPSL LEFKPFSNGP LVGGFVYRGC

    QSERLYGSYV FGDRNGNFLT LQQSPVTKQW QEKPLCLGTS GSCRGYFSGH

    ILGFGEDELG EVYILSSSKS MTQTHNGKLY KIVDPKRPLM PEECRATVQP

    AQTLTSECSR LCRNGYCTPT GKCCCSPGWE GDFCRTAKCE PACRHGGVCV

    RPNKCLCKKG YLGPQCEQVD RNIRRVTRAG ILDQIIDMTS YLLDLTSYIV

  • UCSC uc003ijr.3; human. [Q96QV1-1];


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