Human HSF1 ELISA Kit

Alternative name
Human HSF 1 ELISA Kit;Human heat shock transcription factor 1 ELISA Kit;Human HSTF 1 ELISA Kit;Human HSTF1 ELISA Kit;Human heat shock factor protein 1 ELISA Kit;
Catalog
E018062
species
Human
GeneHSF1
Standard Curve
Other Species Mouse Hsf1 ELISA Kit Bovine HSF1 ELISA Kit Chicken HSF1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Heat Shock Transcription Factor 1 (HSF1). No significant cross-reactivity or interference between Heat Shock Transcription Factor 1 (HSF1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.115ng/mL.
Intended UseHuman HSF1 ELISA Kit allows for the in vitro quantitative determination of HSF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Transcription Factor 1 (HSF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Transcription Factor 1 (HSF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Transcription Factor 1 (HSF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Transcription Factor 1 (HSF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Heat shock factor protein 1 Protein information
Uniprot ID HSF1_HUMAN
Uniprot AC Q00613; A8K4L0; A8MW26; Q53XT4;
UniGene Hs.530227;
GeneID 3297
KEGG hsa:3297;
Human Heat shock factor protein 1 Protein SEQUENCE
SEQUENCE 529 AA; 57260 MW; 735074507C954365 CRC64;
MDLPVGPGAA GPSNVPAFLT KLWTLVSDPD TDALICWSPS GNSFHVFDQG
QFAKEVLPKY FKHNNMASFV RQLNMYGFRK VVHIEQGGLV KPERDDTEFQ
HPCFLRGQEQ LLENIKRKVT SVSTLKSEDI KIRQDSVTKL LTDVQLMKGK
QECMDSKLLA MKHENEALWR EVASLRQKHA QQQKVVNKLI QFLISLVQSN
RILGVKRKIP LMLNDSGSAH SMPKYSRQFS LEHVHGSGPY SAPSPAYSSS
SLYAPDAVAS SGPIISDITE LAPASPMASP GGSIDERPLS SSPLVRVKEE
PPSPPQSPRV EEASPGRPSS VDTLLSPTAL IDSILRESEP APASVTALTD
ARGHTDTEGR PPSPPPTSTP EKCLSVACLD KNELSDHLDA MDSNLDNLQT
MLSSHGFSVD TSALLDLFSP SVTVPDMSLP DLDSSLASIQ ELLSPQEPPR
PPEAENSSPD SGKQLVHYTA QPLFLLDPGS VDTGSNDLPV LFELGEGSYF
SEGDGFAEDP TISLLTGSEP PKAKDPTVS
UCSC uc003zbt.5; human. [Q00613-1];

Related products with Human HSF1 ELISA Kit

Category

Human HSF1 ELISA Kit

Human HSF 1 ELISA Kit;Human heat shock transcription factor 1 ELISA Kit;Human HSTF 1 ELISA Kit;Human HSTF1 ELISA Kit;Human heat shock factor protein 1 ELISA Kit;