Human HSPB8 ELISA Kit

Alternative name
Human HspB8 ELISA Kit;Human alpha-crystallin C chain ELISA Kit;Human E2-induced gene 1 protein ELISA Kit;Human protein kinase H11 ELISA Kit;Human small stress protein-like protein HSP22 ELISA Kit;Human CRYAC ELISA Kit;Human E2IG1 ELISA Kit;Human HSP22 ELISA Kit;Human CMT2L ELISA Kit;Human DHMN2 ELISA Kit;Human H11 ELISA Kit;Human HMN2 ELISA Kit;Human HMN2A ELISA Kit;Human heat shock protein family B (small) member 8 ELISA Kit;Human heat shock protein beta-8 ELISA Kit;Human heat shock 22kDa protein 8 ELISA Kit;Human heat shock 27kDa protein 8 ELISA Kit;
Catalog
E018049
species
Human
GeneHSPB8
Standard Curve
Other Species Mouse Hspb8 ELISA Kit Bovine HSPB8 ELISA Kit Canine HSPB8 ELISA Kit Rat Hspb8 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Heat Shock Protein Beta 8 (HSPb8). No significant cross-reactivity or interference between Heat Shock Protein Beta 8 (HSPb8) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.056ng/mL.
Intended UseHuman HSPB8 ELISA Kit allows for the in vitro quantitative determination of HSPB8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 8 (HSPb8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein Beta 8 (HSPb8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 8 (HSPb8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein Beta 8 (HSPb8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Heat shock protein beta-8 Protein information
Uniprot ID HSPB8_HUMAN
Uniprot AC Q9UJY1; B2R6A6; Q6FIH3; Q9UKS3;
UniGene Hs.400095;
GeneID 26353
KEGG hsa:26353;
Human Heat shock protein beta-8 Protein SEQUENCE
SEQUENCE 196 AA; 21604 MW; B76058CED52292CB CRC64;
MADGQMPFSC HYPSRLRRDP FRDSPLSSRL LDDGFGMDPF PDDLTASWPD
WALPRLSSAW PGTLRSGMVP RGPTATARFG VPAEGRTPPP FPGEPWKVCV
NVHSFKPEEL MVKTKDGYVE VSGKHEEKQQ EGGIVSKNFT KKIQLPAEVD
PVTVFASLSP EGLLIIEAPQ VPPYSTFGES SFNNELPQDS QEVTCT
UCSC uc001txb.4; human.;

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Human HSPB8 ELISA Kit