Human HSP90 Alpha ELISA Kit

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  • Catalog
  • species
  • GeneHSP90 Alpha
  • Other Species Mouse HSP90-alpha ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HSP90a. No significant cross-reactivity or interference between HSP90a and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSP90a and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL
  • Intended UseHuman HSP90 Alpha ELISA Kit allows for the in vitro quantitative determination of HSP90 Alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    Intended Uses: This HSP90a ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HSP90a. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: HSP90a ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-HSP90a antibody and an HSP90a-HRP conjugate. The assay sample and buffer are incubated together with HSP90a-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP90a concentration since HSP90a from samples and HSP90a-HRP conjugate compete for the anti-HSP90a antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP90a from the sample, fewer sites are left to bind HSP90a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP90a concentration in each sample is interpolated from this standard curve.

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