Human HSP72 ELISA Kit

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  • Alternative name

    Heat shock 70 kDa protein 1A/1B ELISA KIT; Heat shock 70 kDa protein 1/2 ELISA KIT; HSP70-1/HSP70-2 ELISA KIT; HSP70.1/HSP70.2HSPA1A ELISA KIT; HSPA1 ELISA KIT; HSX70 HSPA1B ELISA KIT; HSP70-1/HSP70-2 ELISA KIT; HSP70.1/HSP70.2 ELISA KIT

  • Catalog
    E018012
  • species
    Human
  • GeneHSP72
  • Standard CurveHuman HSP72 ELISA Kit
  • Other Species Mouse HSP72 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HSP72. No significant cross-reactivity or interference between HSP72 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSP72 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman HSP72 ELISA Kit allows for the in vitro quantitative determination of HSP72 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This HSP72 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HSP72. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||HSP72 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-HSP72 antibody and an HSP72-HRP conjugate. The assay sample and buffer are incubated together with HSP72-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP72 concentration since HSP72 from samples and HSP72-HRP conjugate compete for the anti-HSP72 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP72 from the sample, fewer sites are left to bind HSP72-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP72 concentration in each sample is interpolated from this standard curve.


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