Human HSF4 ELISA Kit

Alternative name
Human HSF 4 ELISA Kit;Human hHSF4 ELISA Kit;Human heat shock transcription factor 4 ELISA Kit;Human HSTF 4 ELISA Kit;Human CTM ELISA Kit;Human CTRCT5 ELISA Kit;Human heat shock factor protein 4 ELISA Kit;
Catalog
E017981
species
Human
GeneHSF4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman HSF4 ELISA Kit allows for the in vitro quantitative determination of HSF4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human HSF4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HSF4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HSF4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human HSF4 can be calculated.
Human Heat shock factor protein 4 Protein information
Uniprot ID HSF4_HUMAN
Uniprot AC Q9ULV5; Q99472; Q9ULV6;
UniGene Hs.512156; Hs.710714;
GeneID 3299
KEGG hsa:3299;
Human Heat shock factor protein 4 Protein SEQUENCE
SEQUENCE 492 AA; 53011 MW; D5F1C0D68014BC2E CRC64;
MQEAPAALPT EPGPSPVPAF LGKLWALVGD PGTDHLIRWS PSGTSFLVSD
QSRFAKEVLP QYFKHSNMAS FVRQLNMYGF RKVVSIEQGG LLRPERDHVE
FQHPSFVRGR EQLLERVRRK VPALRGDDGR WRPEDLGRLL GEVQALRGVQ
ESTEARLREL RQQNEILWRE VVTLRQSHGQ QHRVIGKLIQ CLFGPLQAGP
SNAGGKRKLS LMLDEGSSCP TPAKFNTCPL PGALLQDPYF IQSPLPETNL
GLSPHRARGP IISDIPEDSP SPEGTRLSPS SDGRREKGLA LLKEEPASPG
GDGEAGLALA PNECDFCVTA PPPLPVAVVQ AILEGKGSFS PEGPRNAQQP
EPGDPREIPD RGPLGLESGD RSPESLLPPM LLQPPQESVE PAGPLDVLGP
SLQGREWTLM DLDMELSLMQ PLVPERGEPE LAVKGLNSPS PGKDPTLGAP
LLLDVQAALG GPALGLPGAL TIYSTPESRT ASYLGPEASP SP
UCSC uc002erl.2; human. [Q9ULV5-1];

Category

Human HSF4 ELISA Kit

Human HSF 4 ELISA Kit;Human hHSF4 ELISA Kit;Human heat shock transcription factor 4 ELISA Kit;Human HSTF 4 ELISA Kit;Human CTM ELISA Kit;Human CTRCT5 ELISA Kit;Human heat shock factor protein 4 ELISA Kit;