Human HSPA5 ELISA Kit

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  • Alternative name

    Human GRP-78 ELISA Kit;Human endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 ELISA Kit;Human Heat shock 70 kDa protein 5 ELISA Kit;Human immunoglobulin heavy chain-binding protein ELISA Kit;Human BIP ELISA Kit;Human GRP78 ELISA Kit;Human HEL-S-89n ELISA Kit;Human MIF2 ELISA Kit;Human heat shock protein family A (Hsp70) member 5 ELISA Kit;Human 78 kDa glucose-regulated protein ELISA Kit;Human epididymis secretory sperm binding protein Li 89n ELISA Kit;Human glucose-regulated protein, 78kDa ELISA Kit;Human heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) ELISA Kit;

  • Catalog
    E017969
  • species
    Human
  • GeneHSPA5
  • Other Species Mouse Hspa5 ELISA KitBovine HSPA5 ELISA KitChicken HSPA5 ELISA KitHorse HSPA5 ELISA KitPorcine HSPA5 ELISA KitRat Hspa5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human HSPA5. No significant cross-reactivity or interference between Human HSPA5 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.039 ng/ml
  • Intended UseHuman HSPA5 ELISA Kit allows for the in vitro quantitative determination of HSPA5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BIP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human 78 kDa glucose-regulated protein Protein information
  • Uniprot ID GRP78_HUMAN
  • Uniprot AC P11021; B0QZ61; Q2EF78; Q9NPF1; Q9UK02;
  • UniGene Hs.743241;
  • GeneID 3309
  • KEGG hsa:3309;
  • Human 78 kDa glucose-regulated protein Protein SEQUENCE
  • SEQUENCE 654 AA; 72333 MW; 59B7D8D85BC32A00 CRC64;

    MKLSLVAAML LLLSAARAEE EDKKEDVGTV VGIDLGTTYS CVGVFKNGRV

    EIIANDQGNR ITPSYVAFTP EGERLIGDAA KNQLTSNPEN TVFDAKRLIG

    RTWNDPSVQQ DIKFLPFKVV EKKTKPYIQV DIGGGQTKTF APEEISAMVL

    TKMKETAEAY LGKKVTHAVV TVPAYFNDAQ RQATKDAGTI AGLNVMRIIN

    EPTAAAIAYG LDKREGEKNI LVFDLGGGTF DVSLLTIDNG VFEVVATNGD

    THLGGEDFDQ RVMEHFIKLY KKKTGKDVRK DNRAVQKLRR EVEKAKRALS

    SQHQARIEIE SFYEGEDFSE TLTRAKFEEL NMDLFRSTMK PVQKVLEDSD

    LKKSDIDEIV LVGGSTRIPK IQQLVKEFFN GKEPSRGINP DEAVAYGAAV

    QAGVLSGDQD TGDLVLLDVC PLTLGIETVG GVMTKLIPRN TVVPTKKSQI

    FSTASDNQPT VTIKVYEGER PLTKDNHLLG TFDLTGIPPA PRGVPQIEVT

    FEIDVNGILR VTAEDKGTGN KNKITITNDQ NRLTPEEIER MVNDAEKFAE

    EDKKLKERID TRNELESYAY SLKNQIGDKE KLGGKLSSED KETMEKAVEE

    KIEWLESHQD ADIEDFKAKK KELEEIVQPI ISKLYGSAGP PPTGEEDTAE

    KDEL



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