Human HSPA1B ELISA Kit

Alternative name
Human heat shock 70 kDa protein 2 ELISA Kit;Human HSP70-2 ELISA Kit;Human HSP70.2 ELISA Kit;Human HSP70-1B ELISA Kit;Human heat shock protein family A (Hsp70) member 1B ELISA Kit;Human heat shock 70 kDa protein 1B ELISA Kit;Human HSP70-1/HSP70-2 ELISA Kit;Human HSP70.1/HSP70.2 ELISA Kit;Human heat shock 70 kDa protein 1/2 ELISA Kit;Human heat shock 70 kDa protein 1A/1B ELISA Kit;Human heat shock 70kD protein 1B ELISA Kit;Human heat shock 70kDa protein 1B ELISA Kit;
Catalog
E017944
species
Human
GeneHSPA1B
SpecificityThis assay has high sensitivity and excellent specificity for detection of HSP701B. No significant cross-reactivity or interference between HSP701B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSP701B and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman HSPA1B ELISA Kit allows for the in vitro quantitative determination of HSPA1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPriciple of the assay: HSP701B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSP701B antibody and an HSP701B-HRP conjugate. The assay sample and buffer are incubated together with HSP701B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP701B concentration since HSP701B from samples and HSP701B-HRP conjugate compete for the anti-HSP701B antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP701B from the sample, fewer sites are left to bind HSP701B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP701B concentration in each sample is interpolated from this standard curve.
Human Heat shock 70 kDa protein 1B Protein information
Uniprot ID HS71B_HUMAN
Uniprot AC P0DMV9; B4E3B6; P08107; P19790; Q5JQI4; Q5SP17; Q9UQL9; Q9UQM0;
UniGene Hs.274402; Hs.702139; Hs.719966; Hs.743411;
GeneID 3304
KEGG hsa:3304;
Human Heat shock 70 kDa protein 1B Protein SEQUENCE
SEQUENCE 641 AA; 70052 MW; 78F513118C96DE66 CRC64;
MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL
IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDK
PKVQVSYKGE TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF
NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL
GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK
KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA
RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL
QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS
LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT
KDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK
ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK
SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE
QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEV D

Category

Human HSPA1B ELISA Kit