Human HSPA1B ELISA Kit

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  • Alternative name

    Human heat shock 70 kDa protein 2 ELISA Kit;Human HSP70-2 ELISA Kit;Human HSP70.2 ELISA Kit;Human HSP70-1B ELISA Kit;Human heat shock protein family A (Hsp70) member 1B ELISA Kit;Human heat shock 70 kDa protein 1B ELISA Kit;Human HSP70-1/HSP70-2 ELISA Kit;Human HSP70.1/HSP70.2 ELISA Kit;Human heat shock 70 kDa protein 1/2 ELISA Kit;Human heat shock 70 kDa protein 1A/1B ELISA Kit;Human heat shock 70kD protein 1B ELISA Kit;Human heat shock 70kDa protein 1B ELISA Kit;

  • Catalog
    E017944
  • species
    Human
  • GeneHSPA1B
  • Standard CurveHuman HSPA1B ELISA Kit
  • Other Species Mouse Hspa1b ELISA KitBovine HSPA1B ELISA KitPorcine HSPA1B ELISA KitRat Hspa1b ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HSP701B. No significant cross-reactivity or interference between HSP701B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HSP701B and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman HSPA1B ELISA Kit allows for the in vitro quantitative determination of HSPA1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Priciple of the assay: HSP701B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSP701B antibody and an HSP701B-HRP conjugate. The assay sample and buffer are incubated together with HSP701B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP701B concentration since HSP701B from samples and HSP701B-HRP conjugate compete for the anti-HSP701B antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP701B from the sample, fewer sites are left to bind HSP701B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP701B concentration in each sample is interpolated from this standard curve.
  • Human Heat shock 70 kDa protein 1B Protein information
  • Uniprot ID HS71B_HUMAN
  • Uniprot AC P0DMV9; B4E3B6; P08107; P19790; Q5JQI4; Q5SP17; Q9UQL9; Q9UQM0;
  • UniGene Hs.274402; Hs.702139; Hs.719966; Hs.743411;
  • GeneID 3304
  • KEGG hsa:3304;
  • Human Heat shock 70 kDa protein 1B Protein SEQUENCE
  • SEQUENCE 641 AA; 70052 MW; 78F513118C96DE66 CRC64;

    MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL

    IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDK

    PKVQVSYKGE TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF

    NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL

    GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK

    KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA

    RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL

    QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS

    LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT

    KDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK

    ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK

    SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE

    QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEV D




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