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Alternative name
WAP four-disulfide core domain protein 2 ELISA KIT; Epididymal secretory protein E4 ELISA KIT; Major epididymis-specific protein E4 ELISA KIT; Putative protease inhibitor WAP5WFDC2 ELISA KIT; HE4 ELISA KIT; WAP5 ELISA KIT
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Catalog
E017911
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species
Human
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GeneHE4
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SpecificityThis kit recognizes natural and recombinant Human HE4. No significant cross-reactivity or interference between Human HE4 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.469pmol/mL
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Intended UseHuman HE4 ELISA Kit allows for the in vitro quantitative determination of HE4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: This ELISA kit applies to the in vitro quantitative determination of Human HE4 concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HE4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HE4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HE4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HE4. You can calculate the concentration of HE4 in the samples by comparing the OD of the samples to the standard curve.
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