Human HBEGF ELISA Kit

Alternative name
Human HB-EGF ELISA Kit;Human HBEGF ELISA Kit;Human Diphtheria toxin receptor ELISA Kit;Human DT-R ELISA Kit;Human DTR ELISA Kit;Human DTS ELISA Kit;Human HEGFL ELISA Kit;Human DTSF ELISA Kit;Human heparin binding EGF like growth factor ELISA Kit;Human proheparin-binding EGF-like growth factor ELISA Kit;Human diphtheria toxin receptor (heparin-binding EGF-like growth factor) ELISA Kit;Human diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) ELISA Kit;Human heparin-binding epidermal growth factor ELISA Kit;
Catalog
E017882
species
Human
GeneHBEGF
SpecificityNatural and recombinant human HBEGF
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman HBEGF ELISA Kit allows for the in vitro quantitative determination of HBEGF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human HBEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HBEGF has been precoated onto 96-well plates. Standards(sf21,D63-L148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HBEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HBEGF amount of sample captured in plate. Background: HBEGF(Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.
Human Heparin-binding EGF-like growth factor Protein information
Uniprot ID HBEGF_HUMAN
Uniprot AC Q99075; B2R821;
UniGene Hs.592942; Hs.799;
GeneID 1839
KEGG hsa:1839;
Human Heparin-binding EGF-like growth factor Protein SEQUENCE
SEQUENCE 208 AA; 23067 MW; 2C43C9D1D8291B51 CRC64;
MKLLPSVVLK LFLAAVLSAL VTGESLERLR RGLAAGTSNP DPPTVSTDQL
LPLGGGRDRK VRDLQEADLD LLRVTLSSKP QALATPNKEE HGKRKKKGKG
LGKKRDPCLR KYKDFCIHGE CKYVKELRAP SCICHPGYHG ERCHGLSLPV
ENRLYTYDHT TILAVVAVVL SSVCLLVIVG LLMFRYHRRG GYDVENEEKV
KLGMTNSH
UCSC uc003lfi.4; human.;

Category

Human HBEGF ELISA Kit