Human SCNN1A ELISA Kit

Alternative name
Human alpha-NaCH ELISA Kit;Human epithelial Na(+) channel subunit alpha ELISA Kit;Human alpha-ENaC ELISA Kit;Human ENaCa ELISA Kit;Human nonvoltage-gated sodium channel 1 subunit alpha ELISA Kit;Human SCNEA ELISA Kit;Human SCNN1 ELISA Kit;Human BESC2 ELISA Kit;Human ENaCalpha ELISA Kit;Human sodium channel epithelial 1 alpha subunit ELISA Kit;Human amiloride-sensitive sodium channel subunit alpha ELISA Kit;Human alpha ENaC-2 ELISA Kit;Human amiloride-sensitive epithelial sodium channel alpha subunit ELISA Kit;Human amiloride-sensitive sodium channel subunit alpha 2 ELISA Kit;Human nasal epithelial sodium channel alpha subunit ELISA Kit;Human sodium channel, non voltage gated 1 alpha subunit ELISA Kit;Human sodium channel, non-voltage-gated 1 alpha ELISA Kit;Human sodium channel, nonvoltage-gated 1 alpha ELISA Kit;
Catalog
E001785
species
Human
GeneSCNN1A
Standard Curve
Other Species Mouse Scnn1a ELISA Kit Bovine SCNN1A ELISA Kit Guinea Pig SCNN1A ELISA Kit Chicken SCNN1A ELISA Kit Rabbit SCNN1A ELISA Kit Rat Scnn1a ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a). No significant cross-reactivity or interference between Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.062ng/mL.
Intended UseHuman SCNN1A ELISA Kit allows for the in vitro quantitative determination of SCNN1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Amiloride-sensitive sodium channel subunit alpha Protein information
Uniprot ID SCNNA_HUMAN
Uniprot AC P37088; A5X2U9; B4E2Q5; C5HTZ0; O43271; Q6GSQ6; Q9UM64;
UniGene Hs.591047;
GeneID 6337
KEGG hsa:6337;
Human Amiloride-sensitive sodium channel subunit alpha Protein SEQUENCE
SEQUENCE 669 AA; 75704 MW; 2CCF342E7DF32E72 CRC64;
MEGNKLEEQD SSPPQSTPGL MKGNKREEQG LGPEPAAPQQ PTAEEEALIE
FHRSYRELFE FFCNNTTIHG AIRLVCSQHN RMKTAFWAVL WLCTFGMMYW
QFGLLFGEYF SYPVSLNINL NSDKLVFPAV TICTLNPYRY PEIKEELEEL
DRITEQTLFD LYKYSSFTTL VAGSRSRRDL RGTLPHPLQR LRVPPPPHGA
RRARSVASSL RDNNPQVDWK DWKIGFQLCN QNKSDCFYQT YSSGVDAVRE
WYRFHYINIL SRLPETLPSL EEDTLGNFIF ACRFNQVSCN QANYSHFHHP
MYGNCYTFND KNNSNLWMSS MPGINNGLSL MLRAEQNDFI PLLSTVTGAR
VMVHGQDEPA FMDDGGFNLR PGVETSISMR KETLDRLGGD YGDCTKNGSD
VPVENLYPSK YTQQVCIHSC FQESMIKECG CAYIFYPRPQ NVEYCDYRKH
SSWGYCYYKL QVDFSSDHLG CFTKCRKPCS VTSYQLSAGY SRWPSVTSQE
WVFQMLSRQN NYTVNNKRNG VAKVNIFFKE LNYKTNSESP SVTMVTLLSN
LGSQWSLWFG SSVLSVVEMA ELVFDLLVIM FLMLLRRFRS RYWSPGRGGR
GAQEVASTLA SSPPSHFCPH PMSLSLSQPG PAPSPALTAP PPAYATLGPR
PSPGGSAGAS SSTCPLGGP
UCSC uc001qnw.3; human. [P37088-1];

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Human SCNN1A ELISA Kit