Human H2AFX ELISA Kit

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  • Alternative name

    Human H2A/X ELISA Kit;Human histone H2A.x ELISA Kit;Human H2AX ELISA Kit;Human H2A.X ELISA Kit;Human H2A histone family member X ELISA Kit;Human histone H2AX ELISA Kit;Human H2AX histone ELISA Kit;

  • Catalog
    E017829
  • species
    Human
  • GeneH2AFX
  • Standard CurveHuman H2AFX ELISA Kit
  • Other Species Mouse H2afx ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of H2A Histone Family, Member X (H2AFX). No significant cross-reactivity or interference between H2A Histone Family, Member X (H2AFX) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman H2AFX ELISA Kit allows for the in vitro quantitative determination of H2AFX , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to H2A Histone Family, Member X (H2AFX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to H2A Histone Family, Member X (H2AFX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H2A Histone Family, Member X (H2AFX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of H2A Histone Family, Member X (H2AFX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Histone H2AX Protein information
  • Uniprot ID H2AX_HUMAN
  • Uniprot AC P16104; Q4ZGJ7; Q6IAS5;
  • UniGene Hs.477879;
  • GeneID 3014
  • KEGG hsa:3014;
  • Human Histone H2AX Protein SEQUENCE
  • SEQUENCE 143 AA; 15145 MW; D4683775C2E6C3A9 CRC64;

    MSGRGKTGGK ARAKAKSRSS RAGLQFPVGR VHRLLRKGHY AERVGAGAPV

    YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGG

    VTIAQGGVLP NIQAVLLPKK TSATVGPKAP SGGKKATQAS QEY

  • UCSC uc001pvg.4; human.;


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