Human H2AFX ELISA Kit

Alternative name
Human H2A/X ELISA Kit;Human histone H2A.x ELISA Kit;Human H2AX ELISA Kit;Human H2A.X ELISA Kit;Human H2A histone family member X ELISA Kit;Human histone H2AX ELISA Kit;Human H2AX histone ELISA Kit;
Catalog
E017829
species
Human
GeneH2AFX
SpecificityThis assay has high sensitivity and excellent specificity for detection of H2A Histone Family, Member X (H2AFX). No significant cross-reactivity or interference between H2A Histone Family, Member X (H2AFX) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.059ng/mL.
Intended UseHuman H2AFX ELISA Kit allows for the in vitro quantitative determination of H2AFX , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to H2A Histone Family, Member X (H2AFX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to H2A Histone Family, Member X (H2AFX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H2A Histone Family, Member X (H2AFX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of H2A Histone Family, Member X (H2AFX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Histone H2AX Protein information
Uniprot ID H2AX_HUMAN
Uniprot AC P16104; Q4ZGJ7; Q6IAS5;
UniGene Hs.477879;
GeneID 3014
KEGG hsa:3014;
Human Histone H2AX Protein SEQUENCE
SEQUENCE 143 AA; 15145 MW; D4683775C2E6C3A9 CRC64;
MSGRGKTGGK ARAKAKSRSS RAGLQFPVGR VHRLLRKGHY AERVGAGAPV
YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGG
VTIAQGGVLP NIQAVLLPKK TSATVGPKAP SGGKKATQAS QEY
UCSC uc001pvg.4; human.;

Human H2AFX ELISA Kit

Human H2A/X ELISA Kit;Human histone H2A.x ELISA Kit;Human H2AX ELISA Kit;Human H2A.X ELISA Kit;Human H2A histone family member X ELISA Kit;Human histone H2AX ELISA Kit;Human H2AX histone ELISA Kit;

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