Human H1F0 ELISA Kit

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  • Alternative name

    Human histone H1' ELISA Kit;Human histone H1(0) ELISA Kit;Human H1FV ELISA Kit;Human H10 ELISA Kit;Human H1 histone family member 0 ELISA Kit;Human histone H1.0 ELISA Kit;Human H1.0, H1(0), H1-0 ELISA Kit;

  • Catalog
    E017819
  • species
    Human
  • GeneH1F0
  • Standard CurveHuman H1F0 ELISA Kit
  • Other Species Bovine H1F0 ELISA KitMouse H1f0 ELISA KitRat H1f0 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of H1 Histone Family, Member 0 (H1F0). No significant cross-reactivity or interference between H1 Histone Family, Member 0 (H1F0) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.34ng/mL.
  • Intended UseHuman H1F0 ELISA Kit allows for the in vitro quantitative determination of H1F0 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to H1 Histone Family, Member 0 (H1F0). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to H1 Histone Family, Member 0 (H1F0). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H1 Histone Family, Member 0 (H1F0), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of H1 Histone Family, Member 0 (H1F0) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Histone H1.0, N-terminally processed Protein information
  • Uniprot ID H10_HUMAN
  • Uniprot AC P07305; B2R6I0; B4DRD6; Q6FG88; Q8N6R3;
  • UniGene Hs.745024;
  • GeneID 3005
  • KEGG hsa:3005;
  • Human Histone H1.0, N-terminally processed Protein SEQUENCE
  • SEQUENCE 194 AA; 20863 MW; 03ED6F01919F8BE1 CRC64;

    MTENSTSAPA AKPKRAKASK KSTDHPKYSD MIVAAIQAEK NRAGSSRQSI

    QKYIKSHYKV GENADSQIKL SIKRLVTTGV LKQTKGVGAS GSFRLAKSDE

    PKKSVAFKKT KKEIKKVATP KKASKPKKAA SKAPTKKPKA TPVKKAKKKL

    AATPKKAKKP KTVKAKPVKA SKPKKAKPVK PKAKSSAKRA GKKK

  • UCSC uc003aty.4; human. [P07305-1];


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