SpecificityThis assay has high sensitivity and excellent specificity for detection of Guanylate Cyclase Activator 2B (GUCA2B).
No significant cross-reactivity or interference between Guanylate Cyclase Activator 2B (GUCA2B) and analogues was observed.
Intended UseHuman GUCA2B ELISA Kit allows for the in vitro quantitative determination of GUCA2B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guanylate Cyclase Activator 2B (GUCA2B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Guanylate Cyclase Activator 2B (GUCA2B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guanylate Cyclase Activator 2B (GUCA2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guanylate Cyclase Activator 2B (GUCA2B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.