Human GUCY1B3 ELISA Kit

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  • Alternative name

    Human GCS-beta-1 ELISA Kit;Human guanylate cyclase soluble subunit beta-3 ELISA Kit;Human GCS-beta-3 ELISA Kit;Human soluble guanylate cyclase small subunit ELISA Kit;Human GUC1B3 ELISA Kit;Human GUCSB3 ELISA Kit;Human GUCY1B1 ELISA Kit;Human GC-S-beta-1 ELISA Kit;Human GC-SB3 ELISA Kit;Human GUCB3 ELISA Kit;Human guanylate cyclase 1 soluble subunit beta ELISA Kit;Human guanylate cyclase soluble subunit beta-1 ELISA Kit;Human guanylate cyclase 1, soluble, beta 3 ELISA Kit;

  • Catalog
    E017771
  • species
    Human
  • GeneGUCY1B3
  • Standard CurveHuman GUCY1B3 ELISA Kit
  • Other Species Mouse Gucy1b3 ELISA KitCanine GUCY1B3 ELISA KitRat Gucy1b3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Guanylate Cyclase 1 Beta 3 (GUCY1b3). No significant cross-reactivity or interference between Guanylate Cyclase 1 Beta 3 (GUCY1b3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman GUCY1B3 ELISA Kit allows for the in vitro quantitative determination of GUCY1B3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guanylate Cyclase 1 Beta 3 (GUCY1b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Guanylate Cyclase 1 Beta 3 (GUCY1b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guanylate Cyclase 1 Beta 3 (GUCY1b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guanylate Cyclase 1 Beta 3 (GUCY1b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Guanylate cyclase soluble subunit beta-1 Protein information
  • Uniprot ID GCYB1_HUMAN
  • Uniprot AC Q02153; B7Z426; Q86WY5;
  • UniGene Hs.77890;
  • GeneID 2983
  • KEGG hsa:2983;
  • Human Guanylate cyclase soluble subunit beta-1 Protein SEQUENCE
  • SEQUENCE 619 AA; 70514 MW; 231E4E660DE02AA1 CRC64;

    MYGFVNHALE LLVIRNYGPE VWEDIKKEAQ LDEEGQFLVR IIYDDSKTYD

    LVAAASKVLN LNAGEILQMF GKMFFVFCQE SGYDTILRVL GSNVREFLQN

    LDALHDHLAT IYPGMRAPSF RCTDAEKGKG LILHYYSERE GLQDIVIGII

    KTVAQQIHGT EIDMKVIQQR NEECDHTQFL IEEKESKEED FYEDLDRFEE

    NGTQESRISP YTFCKAFPFH IIFDRDLVVT QCGNAIYRVL PQLQPGNCSL

    LSVFSLVRPH IDISFHGILS HINTVFVLRS KEGLLDVEKL ECEDELTGTE

    ISCLRLKGQM IYLPEADSIL FLCSPSVMNL DDLTRRGLYL SDIPLHDATR

    DLVLLGEQFR EEYKLTQELE ILTDRLQLTL RALEDEKKKT DTLLYSVLPP

    SVANELRHKR PVPAKRYDNV TILFSGIVGF NAFCSKHASG EGAMKIVNLL

    NDLYTRFDTL TDSRKNPFVY KVETVGDKYM TVSGLPEPCI HHARSICHLA

    LDMMEIAGQV QVDGESVQIT IGIHTGEVVT GVIGQRMPRY CLFGNTVNLT

    SRTETTGEKG KINVSEYTYR CLMSPENSDP QFHLEHRGPV SMKGKKEPMQ

    VWFLSRKNTG TEETKQDDD

  • UCSC uc003ipc.4; human. [Q02153-1];


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