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Human PPAT ELISA Kit

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  • Alternative name

    Human ATASE ELISA Kit;Human glutamine phosphoribosylpyrophosphate amidotransferase ELISA Kit;Human GPAT ELISA Kit;Human PRAT ELISA Kit;Human phosphoribosyl pyrophosphate amidotransferase ELISA Kit;Human amidophosphoribosyltransferase ELISA Kit;Human glutamine PRPP amidotransferase ELISA Kit;Human glutamine phosphoribosylpyrophosphatate amidotransferase ELISA Kit;
  • Catalog
    E001775
  • species
    Human
  • GenePPAT
  • Standard CurveHuman PPAT ELISA Kit
  • Other Species Chicken PPAT ELISA KitRat Ppat ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PPAT. No significant cross-reactivity or interference between PPAT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PPAT and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman PPAT ELISA Kit allows for the in vitro quantitative determination of PPAT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: PPAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PPAT antibody and an PPAT-HRP conjugate. The assay sample and buffer are incubated together with PPAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PPAT concentration since PPAT from samples and PPAT-HRP conjugate compete for the anti-PPAT antibody binding site. Since the number of sites is limited, as more sites are occupied by PPAT from the sample, fewer sites are left to bind PPAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPAT concentration in each sample is interpolated from this standard curve.
  • Human Amidophosphoribosyltransferase Protein information
  • Uniprot ID PUR1_HUMAN
  • Uniprot AC Q06203;
  • UniGene Hs.331420;
  • GeneID 5471
  • KEGG hsa:5471;
  • Human Amidophosphoribosyltransferase Protein SEQUENCE

  • SEQUENCE 517 AA; 57399 MW; D3F2A354C36B29D9 CRC64;

    MELEELGIRE ECGVFGCIAS GEWPTQLDVP HVITLGLVGL QHRGQESAGI

    VTSDGSSVPT FKSHKGMGLV NHVFTEDNLK KLYVSNLGIG HTRYATTGKC

    ELENCQPFVV ETLHGKIAVA HNGELVNAAR LRKKLLRHGI GLSTSSDSEM

    ITQLLAYTPP QEQDDTPDWV ARIKNLMKEA PTAYSLLIMH RDVIYAVRDP

    YGNRPLCIGR LIPVSDINDK EKKTSETEGW VVSSESCSFL SIGARYYREV

    LPGEIVEISR HNVQTLDIIS RSEGNPVAFC IFEYVYFARP DSMFEDQMVY

    TVRYRCGQQL AIEAPVDADL VSTVPESATP AALAYAGKCG LPYVEVLCKN

    RYVGRTFIQP NMRLRQLGVA KKFGVLSDNF KGKRIVLVDD SIVRGNTISP

    IIKLLKESGA KEVHIRVASP PIKYPCFMGI NIPTKEELIA NKPEFDHLAE

    YLGANSVVYL SVEGLVSSVQ EGIKFKKQKE KKHDIMIQEN GNGLECFEKS

    GHCTACLTGK YPVELEW

  • UCSC uc003hbr.4; human.;


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