Human GRB2 ELISA Kit

Alternative name
Human Adapter protein GRB2 ELISA Kit;Human protein Ash ELISA Kit;Human SH2/SH3 adapter GRB2 ELISA Kit;Human ASH ELISA Kit;Human EGFRBP-GRB2 ELISA Kit;Human Grb3-3 ELISA Kit;Human MST084 ELISA Kit;Human MSTP084 ELISA Kit;Human NCKAP2 ELISA Kit;Human growth factor receptor bound protein 2 ELISA Kit;Human growth factor receptor-bound protein 2 ELISA Kit;Human HT027 ELISA Kit;Human abundant SRC homology ELISA Kit;Human epidermal growth factor receptor-binding protein GRB2 ELISA Kit;Human growth factor receptor-bound protein 3 ELISA Kit;
Catalog
E017561
species
Human
GeneGRB2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Growth Factor Receptor Bound Protein 2 (Grb2). No significant cross-reactivity or interference between Growth Factor Receptor Bound Protein 2 (Grb2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.055ng/mL.
Intended UseHuman GRB2 ELISA Kit allows for the in vitro quantitative determination of GRB2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Factor Receptor Bound Protein 2 (Grb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Factor Receptor Bound Protein 2 (Grb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Factor Receptor Bound Protein 2 (Grb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Factor Receptor Bound Protein 2 (Grb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Growth factor receptor-bound protein 2 Protein information
Uniprot ID GRB2_HUMAN
Uniprot AC P62993; P29354; Q14450; Q63057; Q63059;
UniGene Hs.444356;
GeneID 2885
KEGG hsa:2885;
Human Growth factor receptor-bound protein 2 Protein SEQUENCE
SEQUENCE 217 AA; 25206 MW; 83A4B0BA1B248DC4 CRC64;
MEAIAKYDFK ATADDELSFK RGDILKVLNE ECDQNWYKAE LNGKDGFIPK
NYIEMKPHPW FFGKIPRAKA EEMLSKQRHD GAFLIRESES APGDFSLSVK
FGNDVQHFKV LRDGAGKYFL WVVKFNSLNE LVDYHRSTSV SRNQQIFLRD
IEQVPQQPTY VQALFDFDPQ EDGELGFRRG DFIHVMDNSD PNWWKGACHG
QTGMFPRNYV TPVNRNV
UCSC uc002jnx.5; human.;

Human GRB2 ELISA Kit

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