Human GRB10 ELISA Kit

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  • Alternative name

    Human GRB10 adapter protein ELISA Kit;Human insulin receptor-binding protein Grb-IR ELISA Kit;Human GRBIR ELISA Kit;Human KIAA0207 ELISA Kit;Human GRB-IR ELISA Kit;Human Grb-10 ELISA Kit;Human IRBP ELISA Kit;Human MEG1 ELISA Kit;Human RSS ELISA Kit;Human growth factor receptor bound protein 10 ELISA Kit;Human growth factor receptor-bound protein 10 ELISA Kit;Human GRB10 adaptor protein ELISA Kit;Human maternally expressed gene 1 ELISA Kit;

  • Catalog
    E017556
  • species
    Human
  • GeneGRB10
  • Standard CurveHuman GRB10 ELISA Kit
  • Other Species Mouse Grb10 ELISA KitPorcine Grb10 ELISA KitRat Grb10 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Growth Factor Receptor Bound Protein 10 (Grb10). No significant cross-reactivity or interference between Growth Factor Receptor Bound Protein 10 (Grb10) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.102ng/mL.
  • Intended UseHuman GRB10 ELISA Kit allows for the in vitro quantitative determination of GRB10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Factor Receptor Bound Protein 10 (Grb10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Factor Receptor Bound Protein 10 (Grb10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Factor Receptor Bound Protein 10 (Grb10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Factor Receptor Bound Protein 10 (Grb10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Growth factor receptor-bound protein 10 Protein information
  • Uniprot ID GRB10_HUMAN
  • Uniprot AC Q92606; Q92907; Q92948;
  • UniGene Hs.164060;
  • GeneID 2887
  • KEGG hsa:2887;
  • Human Growth factor receptor-bound protein 10 Protein SEQUENCE
  • SEQUENCE 594 AA; 67231 MW; 53A5F885E17C6C6B CRC64;

    MALAGCPDSF LHHPYYQDKV EQTPRSQQDP AGPGLPAQSD RLANHQEDDV

    DLEALVNDMN ASLESLYSAC SMQSDTVPLL QNGQHARSQP RASGPPRSIQ

    PQVSPRQRVQ RSQPVHILAV RRLQEEDQQF RTSSLPAIPN PFPELCGPGS

    PPVLTPGSLP PSQAAAKQDV KVFSEDGTSK VVEILADMTA RDLCQLLVYK

    SHCVDDNSWT LVEHHPHLGL ERCLEDHELV VQVESTMASE SKFLFRKNYA

    KYEFFKNPMN FFPEQMVTWC QQSNGSQTQL LQNFLNSSSC PEIQGFLHVK

    ELGKKSWKKL YVCLRRSGLY CSTKGTSKEP RHLQLLADLE DSNIFSLIAG

    RKQYNAPTDH GLCIKPNKVR NETKELRLLC AEDEQTRTCW MTAFRLLKYG

    MLLYQNYRIP QQRKALLSPF STPVRSVSEN SLVAMDFSGQ TGRVIENPAE

    AQSAALEEGH AWRKRSTRMN ILGSQSPLHP STLSTVIHRT QHWFHGRISR

    EESHRIIKQQ GLVDGLFLLR DSQSNPKAFV LTLCHHQKIK NFQILPCEDD

    GQTFFSLDDG NTKFSDLIQL VDFYQLNKGV LPCKLKHHCI RVAL

  • UCSC uc003tph.4; human. [Q13322-1];



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