Human GDF5 ELISA Kit

Alternative name
Human GDF-5 ELISA Kit;Human bone morphogenetic protein 14 ELISA Kit;Human BMP-14 ELISA Kit;Human Cartilage-derived morphogenetic protein 1 ELISA Kit;Human CDMP-1 ELISA Kit;Human lipopolysaccharide-associated protein 4 ELISA Kit;Human LAP-4 ELISA Kit;Human LPS-associated protein 4 ELISA Kit;Human radotermin ELISA Kit;Human BMP14 ELISA Kit;Human CDMP1 ELISA Kit;Human BDA1C ELISA Kit;Human LAP4 ELISA Kit;Human OS5 ELISA Kit;Human SYM1B ELISA Kit;Human SYNS2 ELISA Kit;Human growth differentiation factor 5 ELISA Kit;Human growth/differentiation factor 5 ELISA Kit;Human cartilage-derived morphogenetic protein-1 ELISA Kit;
Catalog
E017538
species
Human
GeneGDF5
Standard Curve
Other Species Mouse Gdf5 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of GDF-5. No significant cross-reactivity or interference between GDF-5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GDF-5 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman GDF5 ELISA Kit allows for the in vitro quantitative determination of GDF5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCancer
Product Description
specificalPrinciple of the assay: GDF-5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GDF-5 antibody and an GDF-5-HRP conjugate. The assay sample and buffer are incubated together with GDF-5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GDF-5 concentration since GDF-5 from samples and GDF-5-HRP conjugate compete for the anti-GDF-5 antibody binding site. Since the number of sites is limited, as more sites are occupied by GDF-5 from the sample, fewer sites are left to bind GDF-5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF-5 concentration in each sample is interpolated from this standard curve.
Human Growth/differentiation factor 5 Protein information
Uniprot ID GDF5_HUMAN
Uniprot AC P43026; E1P5Q2; Q96SB1;
UniGene Hs.1573;
GeneID 8200
KEGG hsa:8200;
Human Growth/differentiation factor 5 Protein SEQUENCE
SEQUENCE 501 AA; 55411 MW; 37985F2D15C4F5EF CRC64;
MRLPKLLTFL LWYLAWLDLE FICTVLGAPD LGQRPQGTRP GLAKAEAKER
PPLARNVFRP GGHSYGGGAT NANARAKGGT GQTGGLTQPK KDEPKKLPPR
PGGPEPKPGH PPQTRQATAR TVTPKGQLPG GKAPPKAGSV PSSFLLKKAR
EPGPPREPKE PFRPPPITPH EYMLSLYRTL SDADRKGGNS SVKLEAGLAN
TITSFIDKGQ DDRGPVVRKQ RYVFDISALE KDGLLGAELR ILRKKPSDTA
KPAAPGGGRA AQLKLSSCPS GRQPASLLDV RSVPGLDGSG WEVFDIWKLF
RNFKNSAQLC LELEAWERGR AVDLRGLGFD RAARQVHEKA LFLVFGRTKK
RDLFFNEIKA RSGQDDKTVY EYLFSQRRKR RAPLATRQGK RPSKNLKARC
SRKALHVNFK DMGWDDWIIA PLEYEAFHCE GLCEFPLRSH LEPTNHAVIQ
TLMNSMDPES TPPTCCVPTR LSPISILFID SANNVVYKQY EDMVVESCGC
R
UCSC uc002xck.2; human.;

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Human GDF5 ELISA Kit