Human GZMM ELISA Kit

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  • Alternative name

    Human Met-1 serine protease ELISA Kit;Human HU-Met-1 ELISA Kit;Human met-ase ELISA Kit;Human natural killer cell granular protease ELISA Kit;Human MET1 ELISA Kit;Human LMET1 ELISA Kit;Human granzyme M ELISA Kit;Human lymphocyte met-ase 1 ELISA Kit;

  • Catalog
    E017448
  • species
    Human
  • GeneGZMM
  • Standard CurveHuman GZMM ELISA Kit
  • Other Species Mouse GZMM ELISA KitRat Gzmm ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Granzyme M (GZMM). No significant cross-reactivity or interference between Granzyme M (GZMM) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.3pg/mL.
  • Intended UseHuman GZMM ELISA Kit allows for the in vitro quantitative determination of GZMM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granzyme M (GZMM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Granzyme M (GZMM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granzyme M (GZMM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Granzyme M (GZMM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Granzyme M Protein information
  • Uniprot ID GRAM_HUMAN
  • Uniprot AC P51124;
  • UniGene Hs.465511;
  • GeneID 3004
  • KEGG hsa:3004;
  • Human Granzyme M Protein SEQUENCE
  • SEQUENCE 257 AA; 27545 MW; B4E815CE455F7371 CRC64;

    MEACVSSLLV LALGALSVGS SFGTQIIGGR EVIPHSRPYM ASLQRNGSHL

    CGGVLVHPKW VLTAAHCLAQ RMAQLRLVLG LHTLDSPGLT FHIKAAIQHP

    RYKPVPALEN DLALLQLDGK VKPSRTIRPL ALPSKRQVVA AGTRCSMAGW

    GLTHQGGRLS RVLRELDLQV LDTRMCNNSR FWNGSLSPSM VCLAADSKDQ

    APCKGDSGGP LVCGKGRVLA RVLSFSSRVC TDIFKPPVAT AVAPYVSWIR

    KVTGRSA

  • UCSC uc002low.3; human.;


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