Human IL6ST ELISA Kit

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  • Alternative name

    Human IL-6 receptor subunit beta ELISA Kit;Human IL-6R subunit beta ELISA Kit;Human IL-6R-beta ELISA Kit;Human IL-6RB ELISA Kit;Human CDW130 ELISA Kit;Human Interleukin-6 signal transducer ELISA Kit;Human membrane glycoprotein 130 ELISA Kit;Human GP130 ELISA Kit;Human oncostatin-M receptor subunit alpha ELISA Kit;Human CD130 ELISA Kit;Human interleukin 6 signal transducer ELISA Kit;Human interleukin-6 receptor subunit beta ELISA Kit;Human CD130 antigen ELISA Kit;Human gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form ELISA Kit;Human gp130, oncostatin M receptor ELISA Kit;Human interleukin receptor beta chain ELISA Kit;Human membrane glycoprotein gp130 ELISA Kit;

  • Catalog
    E017343
  • species
    Human
  • GeneIL6ST
  • Other Species Human Gp130/IL6ST ELISA KitMouse Il6st ELISA KitMouse Gp130/IL6ST ELISA KitRat Il6st ELISA Kit
  • SpecificityNatural and recombinant human sGP130
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman IL6ST ELISA Kit allows for the in vitro quantitative determination of IL6ST , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human GP130 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GP130 has been precoated onto 96-well plates. Standards(sf21,L24-E619) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GP130 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GP130 amount of sample captured in plate. Background: GP130(Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound gp130. Gp130 is an important part of many different types of signaling complexes.Gp130 molecules with mutations in either of these segments could not transduce a growth signal.
  • Human Interleukin-6 receptor subunit beta Protein information
  • Uniprot ID IL6RB_HUMAN
  • Uniprot AC P40189; A0N0L4; Q5FC04; Q9UQ41;
  • UniGene Hs.532082; Hs.706627;
  • GeneID 3572
  • KEGG hsa:3572;
  • Human Interleukin-6 receptor subunit beta Protein SEQUENCE
  • SEQUENCE 918 AA; 103537 MW; 6510A4409FFCF08C CRC64;

    MLTLQTWLVQ ALFIFLTTES TGELLDPCGY ISPESPVVQL HSNFTAVCVL

    KEKCMDYFHV NANYIVWKTN HFTIPKEQYT IINRTASSVT FTDIASLNIQ

    LTCNILTFGQ LEQNVYGITI ISGLPPEKPK NLSCIVNEGK KMRCEWDGGR

    ETHLETNFTL KSEWATHKFA DCKAKRDTPT SCTVDYSTVY FVNIEVWVEA

    ENALGKVTSD HINFDPVYKV KPNPPHNLSV INSEELSSIL KLTWTNPSIK

    SVIILKYNIQ YRTKDASTWS QIPPEDTAST RSSFTVQDLK PFTEYVFRIR

    CMKEDGKGYW SDWSEEASGI TYEDRPSKAP SFWYKIDPSH TQGYRTVQLV

    WKTLPPFEAN GKILDYEVTL TRWKSHLQNY TVNATKLTVN LTNDRYLATL

    TVRNLVGKSD AAVLTIPACD FQATHPVMDL KAFPKDNMLW VEWTTPRESV

    KKYILEWCVL SDKAPCITDW QQEDGTVHRT YLRGNLAESK CYLITVTPVY

    ADGPGSPESI KAYLKQAPPS KGPTVRTKKV GKNEAVLEWD QLPVDVQNGF

    IRNYTIFYRT IIGNETAVNV DSSHTEYTLS SLTSDTLYMV RMAAYTDEGG

    KDGPEFTFTT PKFAQGEIEA IVVPVCLAFL LTTLLGVLFC FNKRDLIKKH

    IWPNVPDPSK SHIAQWSPHT PPRHNFNSKD QMYSDGNFTD VSVVEIEAND

    KKPFPEDLKS LDLFKKEKIN TEGHSSGIGG SSCMSSSRPS ISSSDENESS

    QNTSSTVQYS TVVHSGYRHQ VPSVQVFSRS ESTQPLLDSE ERPEDLQLVD

    HVDGGDGILP RQQYFKQNCS QHESSPDISH FERSKQVSSV NEEDFVRLKQ

    QISDHISQSC GSGQMKMFQE VSAADAFGPG TEGQVERFET VGMEAATDEG

    MPKSYLPQTV RQGGYMPQ

  • UCSC uc003jqq.4; human. [P40189-1];


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