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Human GPC1 ELISA Kit

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  • Alternative name

    Human glypican ELISA Kit;Human glypican 1 ELISA Kit;Human glypican-1 ELISA Kit;Human glypican proteoglycan 1 ELISA Kit;
  • Catalog
    E017211
  • species
    Human
  • GeneGPC1
  • Standard CurveHuman GPC1 ELISA Kit
  • Other Species Mouse Gpc1 ELISA KitBovine GPC1 ELISA KitRat Gpc1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GPC-1. No significant cross-reactivity or interference between GPC-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete thecross-reactivity detection between GPC-1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman GPC1 ELISA Kit allows for the in vitro quantitative determination of GPC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the assay: GPC-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPC-1 antibody and an GPC-1-HRP conjugate. The assay sample and buffer are incubated together with GPC-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPC-1 concentration since GPC-1 from samples and GPC-1-HRP conjugate compete for the anti-GPC-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPC-1 from the sample, fewer sites are left to bind GPC-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPC-1 concentration in each sample is interpolated from this standard curve.
  • Human Secreted glypican-1 Protein information
  • Uniprot ID GPC1_HUMAN
  • Uniprot AC P35052; B3KTD1; Q53QM4;
  • UniGene Hs.328232;
  • GeneID 2817
  • KEGG hsa:2817;
  • Human Secreted glypican-1 Protein SEQUENCE

  • SEQUENCE 558 AA; 61680 MW; 16553B56080A83C8 CRC64;

    MELRARGWWL LCAAAALVAC ARGDPASKSR SCGEVRQIYG AKGFSLSDVP

    QAEISGEHLR ICPQGYTCCT SEMEENLANR SHAELETALR DSSRVLQAML

    ATQLRSFDDH FQHLLNDSER TLQATFPGAF GELYTQNARA FRDLYSELRL

    YYRGANLHLE ETLAEFWARL LERLFKQLHP QLLLPDDYLD CLGKQAEALR

    PFGEAPRELR LRATRAFVAA RSFVQGLGVA SDVVRKVAQV PLGPECSRAV

    MKLVYCAHCL GVPGARPCPD YCRNVLKGCL ANQADLDAEW RNLLDSMVLI

    TDKFWGTSGV ESVIGSVHTW LAEAINALQD NRDTLTAKVI QGCGNPKVNP

    QGPGPEEKRR RGKLAPRERP PSGTLEKLVS EAKAQLRDVQ DFWISLPGTL

    CSEKMALSTA SDDRCWNGMA RGRYLPEVMG DGLANQINNP EVEVDITKPD

    MTIRQQIMQL KIMTNRLRSA YNGNDVDFQD ASDDGSGSGS GDGCLDDLCS

    RKVSRKSSSS RTPLTHALPG LSEQEGQKTS AASCPQPPTF LLPLLLFLAL

    TVARPRWR

  • UCSC uc002vyw.5; human. [P35052-1];


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