Human PYGM ELISA Kit

Alternative name
Human myophosphorylase ELISA Kit;Human glycogen phosphorylase, muscle associated ELISA Kit;Human glycogen phosphorylase, muscle form ELISA Kit;Human phosphorylase, glycogen, muscle ELISA Kit;
Catalog
E017070
species
Human
GenePYGM
Standard Curve
Other Species Mouse Pygm ELISA Kit Bovine PYGM ELISA Kit Monkey PYGM ELISA Kit Rabbit PYGM ELISA Kit Rat Pygm ELISA Kit Sheep PYGM ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glycogen Phosphorylase, Muscle (PYGM). No significant cross-reactivity or interference between Glycogen Phosphorylase, Muscle (PYGM) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.127ng/mL.
Intended UseHuman PYGM ELISA Kit allows for the in vitro quantitative determination of PYGM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen Phosphorylase, Muscle (PYGM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycogen Phosphorylase, Muscle (PYGM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycogen Phosphorylase, Muscle (PYGM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycogen Phosphorylase, Muscle (PYGM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Glycogen phosphorylase, muscle form Protein information
Uniprot ID PYGM_HUMAN
Uniprot AC P11217; A0AVK1; A6NDY6;
UniGene Hs.154084;
GeneID 5837
KEGG hsa:5837;
Human Glycogen phosphorylase, muscle form Protein SEQUENCE
SEQUENCE 842 AA; 97092 MW; EBDB7D80D740B68F CRC64;
MSRPLSDQEK RKQISVRGLA GVENVTELKK NFNRHLHFTL VKDRNVATPR
DYYFALAHTV RDHLVGRWIR TQQHYYEKDP KRIYYLSLEF YMGRTLQNTM
VNLALENACD EATYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT
LGLAAYGYGI RYEFGIFNQK ISGGWQMEEA DDWLRYGNPW EKARPEFTLP
VHFYGHVEHT SQGAKWVDTQ VVLAMPYDTP VPGYRNNVVN TMRLWSAKAP
NDFNLKDFNV GGYIQAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV
VAATLQDIIR RFKSSKFGCR DPVRTNFDAF PDKVAIQLND THPSLAIPEL
MRILVDLERM DWDKAWDVTV RTCAYTNHTV LPEALERWPV HLLETLLPRH
LQIIYEINQR FLNRVAAAFP GDVDRLRRMS LVEEGAVKRI NMAHLCIAGS
HAVNGVARIH SEILKKTIFK DFYELEPHKF QNKTNGITPR RWLVLCNPGL
AEVIAERIGE DFISDLDQLR KLLSFVDDEA FIRDVAKVKQ ENKLKFAAYL
EREYKVHINP NSLFDIQVKR IHEYKRQLLN CLHVITLYNR IKREPNKFFV
PRTVMIGGKA APGYHMAKMI IRLVTAIGDV VNHDPAVGDR LRVIFLENYR
VSLAEKVIPA ADLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM
AEEAGEENFF IFGMRVEDVD KLDQRGYNAQ EYYDRIPELR QVIEQLSSGF
FSPKQPDLFK DIVNMLMHHD RFKVFADYED YIKCQEKVSA LYKNPREWTR
MVIRNIATSG KFSSDRTIAQ YAREIWGVEP SRQRLPAPDE AI
UCSC uc001oax.5; human. [P11217-1];

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Human PYGM ELISA Kit

Human myophosphorylase ELISA Kit;Human glycogen phosphorylase, muscle associated ELISA Kit;Human glycogen phosphorylase, muscle form ELISA Kit;Human phosphorylase, glycogen, muscle ELISA Kit;