Human PYGL ELISA Kit

Alternative name
Human GSD6 ELISA Kit;Human glycogen phosphorylase L ELISA Kit;Human glycogen phosphorylase, liver form ELISA Kit;Human phosphorylase, glycogen, liver ELISA Kit;
Catalog
E017067
species
Human
GenePYGL
Other Species Mouse Pygl ELISA Kit Bovine PYGL ELISA Kit Rat Pygl ELISA Kit Sheep PYGL ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman PYGL ELISA Kit allows for the in vitro quantitative determination of PYGL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: PYGL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PYGL antibody and an PYGL-HRP conjugate. The assay sample and buffer are incubated together with PYGL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PYGL concentration since PYGL from samples and PYGL-HRP conjugate compete for the anti-PYGL antibody binding site. Since the number of sites is limited, as more sites are occupied by PYGL from the sample, fewer sites are left to bind PYGL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PYGL concentration in each sample is interpolated from this standard curve.
Human Glycogen phosphorylase, liver form Protein information
Uniprot ID PYGL_HUMAN
Uniprot AC Q641R5; Q96G82;
UniGene Hs.282417;
GeneID 5836
KEGG hsa:5836;
Human Glycogen phosphorylase, liver form Protein SEQUENCE
SEQUENCE 847 AA; 97149 MW; 74017E8125FB5735 CRC64;
MAKPLTDQEK RRQISIRGIV GVENVAELKK SFNRHLHFTL VKDRNVATTR
DYYFALAHTV RDHLVGRWIR TQQHYYDKCP KRVYYLSLEF YMGRTLQNTM
INLGLQNACD EAIYQLGLDI EELEEIEEDA GLGNGGLGRL AACFLDSMAT
LGLAAYGYGI RYEYGIFNQK IRDGWQVEEA DDWLRYGNPW EKSRPEFMLP
VHFYGKVEHT NTGTKWIDTQ VVLALPYDTP VPGYMNNTVN TMRLWSARAP
NDFNLRDFNV GDYIQAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV
VAATLQDIIR RFKASKFGST RGAGTVFDAF PDQVAIQLND THPALAIPEL
MRIFVDIEKL PWSKAWELTQ KTFAYTNHTV LPEALERWPV DLVEKLLPRH
LEIIYEINQK HLDRIVALFP KDVDRLRRMS LIEEEGSKRI NMAHLCIVGS
HAVNGVAKIH SDIVKTKVFK DFSELEPDKF QNKTNGITPR RWLLLCNPGL
AELIAEKIGE DYVKDLSQLT KLHSFLGDDV FLRELAKVKQ ENKLKFSQFL
ETEYKVKINP SSMFDVQVKR IHEYKRQLLN CLHVITMYNR IKKDPKKLFV
PRTVIIGGKA APGYHMAKMI IKLITSVADV VNNDPMVGSK LKVIFLENYR
VSLAEKVIPA TDLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM
AEEAGEENLF IFGMRIDDVA ALDKKGYEAK EYYEALPELK LVIDQIDNGF
FSPKQPDLFK DIINMLFYHD RFKVFADYEA YVKCQDKVSQ LYMNPKAWNT
MVLKNIAASG KFSSDRTIKE YAQNIWNVEP SDLKISLSNE SNKVNGN
UCSC uc001wyu.4; human. [P06737-1];

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Human PYGL ELISA Kit

Human GSD6 ELISA Kit;Human glycogen phosphorylase L ELISA Kit;Human glycogen phosphorylase, liver form ELISA Kit;Human phosphorylase, glycogen, liver ELISA Kit;