Human GLDC ELISA Kit

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  • Alternative name

    Human Glycine cleavage system P protein ELISA Kit;Human glycine decarboxylase ELISA Kit;Human glycine dehydrogenase (aminomethyl-transferring) ELISA Kit;Human GCSP ELISA Kit;Human GCE ELISA Kit;Human HYGN1 ELISA Kit;Human glycine dehydrogenase (decarboxylating), mitochondrial ELISA Kit;Human glycine cleavage system protein P ELISA Kit;Human glycine decarboxylase P-protein ELISA Kit;Human glycine dehydrogenase [decarboxylating], mitochondrial ELISA Kit;

  • Catalog
    E017015
  • species
    Human
  • GeneGLDC
  • Standard CurveHuman GLDC ELISA Kit
  • Other Species Mouse Gldc ELISA KitChicken GLDC ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Glycine Dehydrogenase (GLDC). No significant cross-reactivity or interference between Glycine Dehydrogenase (GLDC) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.132ng/mL.
  • Intended UseHuman GLDC ELISA Kit allows for the in vitro quantitative determination of GLDC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine Dehydrogenase (GLDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycine Dehydrogenase (GLDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycine Dehydrogenase (GLDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycine Dehydrogenase (GLDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Glycine dehydrogenase (decarboxylating), mitochondrial Protein information
  • Uniprot ID GCSP_HUMAN
  • Uniprot AC P23378; Q2M2F8;
  • UniGene Hs.584238;
  • GeneID 2731
  • KEGG hsa:2731;
  • Human Glycine dehydrogenase (decarboxylating), mitochondrial Protein SEQUENCE
  • SEQUENCE 1020 AA; 112730 MW; 8FAEA7D56BEB17B2 CRC64;

    MQSCARAWGL RLGRGVGGGR RLAGGSGPCW APRSRDSSSG GGDSAAAGAS

    RLLERLLPRH DDFARRHIGP GDKDQREMLQ TLGLASIDEL IEKTVPANIR

    LKRPLKMEDP VCENEILATL HAISSKNQIW RSYIGMGYYN CSVPQTILRN

    LLENSGWITQ YTPYQPEVSQ GRLESLLNYQ TMVCDITGLD MANASLLDEG

    TAAAEALQLC YRHNKRRKFL VDPRCHPQTI AVVQTRAKYT GVLTELKLPC

    EMDFSGKDVS GVLFQYPDTE GKVEDFTELV ERAHQSGSLA CCATDLLALC

    ILRPPGEFGV DIALGSSQRF GVPLGYGGPH AAFFAVRESL VRMMPGRMVG

    VTRDATGKEV YRLALQTREQ HIRRDKATSN ICTAQALLAN MAAMFAIYHG

    SHGLEHIARR VHNATLILSE GLKRAGHQLQ HDLFFDTLKI QCGCSVKEVL

    GRAAQRQINF RLFEDGTLGI SLDETVNEKD LDDLLWIFGC ESSAELVAES

    MGEECRGIPG SVFKRTSPFL THQVFNSYHS ETNIVRYMKK LENKDISLVH

    SMIPLGSCTM KLNSSSELAP ITWKEFANIH PFVPLDQAQG YQQLFRELEK

    DLCELTGYDQ VCFQPNSGAQ GEYAGLATIR AYLNQKGEGH RTVCLIPKSA

    HGTNPASAHM AGMKIQPVEV DKYGNIDAVH LKAMVDKHKE NLAAIMITYP

    STNGVFEENI SDVCDLIHQH GGQVYLDGAN MNAQVGICRP GDFGSDVSHL

    NLHKTFCIPH GGGGPGMGPI GVKKHLAPFL PNHPVISLKR NEDACPVGTV

    SAAPWGSSSI LPISWAYIKM MGGKGLKQAT ETAILNANYM AKRLETHYRI

    LFRGARGYVG HEFILDTRPF KKSANIEAVD VAKRLQDYGF HAPTMSWPVA

    GTLMVEPTES EDKAELDRFC DAMISIRQEI ADIEEGRIDP RVNPLKMSPH

    SLTCVTSSHW DRPYSREVAA FPLPFVKPEN KFWPTIARID DIYGDQHLVC

    TCPPMEVYES PFSEQKRASS

  • UCSC uc003zkc.4; human.;


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