Human GPAM ELISA Kit

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  • Alternative name

    Human GPAT-1 ELISA Kit;Human GPAT1 ELISA Kit;Human KIAA1560 ELISA Kit;Human GPAT ELISA Kit;Human glycerol-3-phosphate acyltransferase, mitochondrial ELISA Kit;Human glycerol-3-phosphate acyltransferase 1, mitochondrial ELISA Kit;

  • Catalog
    E016991
  • species
    Human
  • GeneGPAM
  • Standard CurveHuman GPAM ELISA Kit
  • Other Species Mouse Gpam ELISA KitBovine GPAM ELISA KitPorcine GPAM ELISA KitRat Gpam ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM). No significant cross-reactivity or interference between Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.55ng/mL.
  • Intended UseHuman GPAM ELISA Kit allows for the in vitro quantitative determination of GPAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Glycerol-3-phosphate acyltransferase 1, mitochondrial Protein information
  • Uniprot ID GPAT1_HUMAN
  • Uniprot AC Q9HCL2; Q5VW51; Q86TA3;
  • UniGene Hs.42586;
  • GeneID 57678
  • KEGG hsa:57678;
  • Human Glycerol-3-phosphate acyltransferase 1, mitochondrial Protein SEQUENCE
  • SEQUENCE 828 AA; 93795 MW; 7AADD21BC8684693 CRC64;

    MDESALTLGT IDVSYLPHSS EYSVGRCKHT SEEWGECGFR PTIFRSATLK

    WKESLMSRKR PFVGRCCYSC TPQSWDKFFN PSIPSLGLRN VIYINETHTR

    HRGWLARRLS YVLFIQERDV HKGMFATNVT ENVLNSSRVQ EAIAEVAAEL

    NPDGSAQQQS KAVNKVKKKA KRILQEMVAT VSPAMIRLTG WVLLKLFNSF

    FWNIQIHKGQ LEMVKAATET NLPLLFLPVH RSHIDYLLLT FILFCHNIKA

    PYIASGNNLN IPIFSTLIHK LGGFFIRRRL DETPDGRKDV LYRALLHGHI

    VELLRQQQFL EIFLEGTRSR SGKTSCARAG LLSVVVDTLS TNVIPDILII

    PVGISYDRII EGHYNGEQLG KPKKNESLWS VARGVIRMLR KNYGCVRVDF

    AQPFSLKEYL ESQSQKPVSA LLSLEQALLP AILPSRPSDA ADEGRDTSIN

    ESRNATDESL RRRLIANLAE HILFTASKSC AIMSTHIVAC LLLYRHRQGI

    DLSTLVEDFF VMKEEVLARD FDLGFSGNSE DVVMHAIQLL GNCVTITHTS

    RNDEFFITPS TTVPSVFELN FYSNGVLHVF IMEAIIACSL YAVLNKRGLG

    GPTSTPPNLI SQEQLVRKAA SLCYLLSNEG TISLPCQTFY QVCHETVGKF

    IQYGILTVAE HDDQEDISPS LAEQQWDKKL PEPLSWRSDE EDEDSDFGEE

    QRDCYLKVSQ SKEHQQFITF LQRLLGPLLE AYSSAAIFVH NFSGPVPEPE

    YLQKLHKYLI TRTERNVAVY AESATYCLVK NAVKMFKDIG VFKETKQKRV

    SVLELSSTFL PQCNRQKLLE YILSFVVL

  • UCSC uc001kzp.4; human.;



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