Human GSTA5 ELISA Kit

Alternative name
Human GST class-alpha member 5 ELISA Kit;Human glutathione S-transferase A5-5 ELISA Kit;Human glutathione S-transferase alpha 5 ELISA Kit;Human glutathione S-transferase A5 ELISA Kit;Human glutathione transferase A5 ELISA Kit;
Catalog
E016916
species
Human
GeneGSTA5
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Alpha 5 (GSTa5). No significant cross-reactivity or interference between Glutathione S Transferase Alpha 5 (GSTa5) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.235ng/mL.
Intended UseHuman GSTA5 ELISA Kit allows for the in vitro quantitative determination of GSTA5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Alpha 5 (GSTa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Alpha 5 (GSTa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Alpha 5 (GSTa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione S Transferase Alpha 5 (GSTa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Glutathione S-transferase A5 Protein information
Uniprot ID GSTA5_HUMAN
Uniprot AC Q7RTV2; Q5SZC2;
UniGene Hs.646984;
GeneID 221357
KEGG hsa:221357;
Human Glutathione S-transferase A5 Protein SEQUENCE
SEQUENCE 222 AA; 25722 MW; 6DFCECF202F2D898 CRC64;
MAEKPKLHYS NARGSMESIR WLLAAAGVEL EEKFLESAED LDKLRNDGSL
LFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDMKERA LIDMYTEGIV
DLTEMILLLL ICQPEERDAK TALVKEKIKN RYFPAFEKVL KSHRQDYLVG
NKLSWADIHL VELFYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP
GSQRKPPMDE KSLEEARKIF RF
UCSC uc003pba.2; human.;

Category

Human GSTA5 ELISA Kit

Human GST class-alpha member 5 ELISA Kit;Human glutathione S-transferase A5-5 ELISA Kit;Human glutathione S-transferase alpha 5 ELISA Kit;Human glutathione S-transferase A5 ELISA Kit;Human glutathione transferase A5 ELISA Kit;