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Alternative name
Glutathione ELISA Kit
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Catalog
E016873
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species
Human
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GeneGSH
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Other Species
Human GSH ELISA KitHuman GSH Px ELISA KitHuman GSH-Px ELISA KitMouse GSH-Px ELISA KitMouse GSH ELISA KitBovine GSH ELISA KitCanine GSH ELISA KitChicken GSH ELISA KitGeneral GSH ELISA KitPorcine GSH ELISA KitRabbit GSH ELISA KitRat GSH ELISA KitCamel GSH ELISA KitGoat GSH ELISA KitGuinea Pig GSH ELISA KitHamster GSH ELISA KitHorse GSH ELISA KitMonkey GSH ELISA KitSheep GSH ELISA Kit
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SpecificityNo cross-reaction with other factors
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.55ug/ml
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Intended UseHuman GSH ELISA Kit allows for the in vitro quantitative determination of GSH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalHuman GSH ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to GSH , During the reaction,GSH in the sample or standard competes with a fixed amount of biotin-labeled GSH for sites on a pre-coated Monoclonal antibody specific to GSH . Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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