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Alternative name
Human thioltransferase-1 ELISA Kit;Human TTase-1 ELISA Kit;Human GRX ELISA Kit;Human GRX1 ELISA Kit;Human glutaredoxin ELISA Kit;Human glutaredoxin-1 ELISA Kit;Human thioltransferase ELISA Kit;
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Catalog
E016855
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species
Human
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GeneGLRX
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Standard Curve
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Other Species
Mouse Glrx ELISA KitBovine GLRX ELISA KitChicken GLRX ELISA KitPorcine GLRX ELISA KitRabbit GLRX ELISA KitRat Glrx ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Glutaredoxin (GLRX).
No significant cross-reactivity or interference between Glutaredoxin (GLRX) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 28.9pg/mL.
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Intended UseHuman GLRX ELISA Kit allows for the in vitro quantitative determination of GLRX , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutaredoxin (GLRX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutaredoxin (GLRX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutaredoxin (GLRX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutaredoxin (GLRX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Human Glutaredoxin-1 Protein information
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Uniprot ID
GLRX1_HUMAN
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Uniprot AC
P35754; B2R4L2; Q3KQS1; Q6ICT1;
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UniGene
Hs.28988;
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GeneID
2745
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KEGG
hsa:2745;
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Human Glutaredoxin-1 Protein SEQUENCE
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SEQUENCE 106 AA; 11776 MW; BB86FED55967EBE2 CRC64;
MAQEFVNCKI QPGKVVVFIK PTCPYCRRAQ EILSQLPIKQ GLLEFVDITA
TNHTNEIQDY
LQQLTGARTV PRVFIGKDCI GGCSDLVSLQ QSGELLTRLK
QIGALQ
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UCSC
uc003kln.5; human.;
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