Human alpha-SMA ELISA Kit

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  • Alternative name

    Actin, gamma-enteric smooth muscle ELISA KIT; Alpha-actin-3 ELISA KIT; Gamma-2-actin ELISA KIT; Smooth muscle gamma-actinACTG2 ELISA KIT; ACTA3 ELISA KIT; ACTL3 ELISA KIT; ACTSG ELISA KIT

  • Catalog
    E001684
  • species
    Human
  • Genealpha-SMA
  • Standard CurveHuman alpha-SMA ELISA Kit
  • Other Species Mouse alpha-SMA ELISA Kit
  • SpecificityNo cross-reaction with other factors.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.06 ng/ml.
  • Intended UseHuman alpha-SMA ELISA Kit allows for the in vitro quantitative determination of alpha-SMA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human alpha-SMA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.


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