Human GPT ELISA Kit

Alternative name
Human ALT1 ELISA Kit;Human glutamate pyruvate transaminase 1 ELISA Kit;Human GPT 1 ELISA Kit;Human Glutamic--alanine transaminase 1 ELISA Kit;Human glutamic--pyruvic transaminase 1 ELISA Kit;Human AAT1 ELISA Kit;Human GPT1 ELISA Kit;Human glutamic--pyruvic transaminase ELISA Kit;Human alanine aminotransferase 1 ELISA Kit;Human alanine aminotransferase ELISA Kit;Human glutamic-alanine transaminase 1 ELISA Kit;Human glutamic-pyruvate transaminase (alanine aminotransferase) ELISA Kit;
Catalog
E016817
species
Human
GeneGPT
SpecificityThis assay has high sensitivity and excellent specificity for detection of human ALT. No significant cross-reactivity or interference between human ALT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity3.9 uIU/ml.
Intended UseHuman GPT ELISA Kit allows for the in vitro quantitative determination of GPT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Alanine aminotransferase 1 Protein information
Uniprot ID ALAT1_HUMAN
Uniprot AC P24298; B0YJ18; D3DWM7; P78398; Q93076;
UniGene Hs.103502;
GeneID 2875
KEGG hsa:2875;
Human Alanine aminotransferase 1 Protein SEQUENCE
SEQUENCE 496 AA; 54637 MW; 23705E7A3A4283B6 CRC64;
MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ
ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF
PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD
PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG
AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ
TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY
AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP
PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA
PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC
VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS
UCSC uc003zdh.5; human.;

Category

Human GPT ELISA Kit