Human GRIA3 ELISA Kit

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  • Alternative name

    Human gluR-3 ELISA Kit;Human AMPA-selective glutamate receptor 3 ELISA Kit;Human GLUR-C ELISA Kit;Human GLUR-K3 ELISA Kit;Human Glutamate receptor ionotropic, AMPA 3 ELISA Kit;Human GluA3 ELISA Kit;Human GLUR3 ELISA Kit;Human GLURC ELISA Kit;Human MRX94 ELISA Kit;Human glutamate ionotropic receptor AMPA type subunit 3 ELISA Kit;Human glutamate receptor 3 ELISA Kit;Human dJ1171F9.1 ELISA Kit;Human glutamate receptor C ELISA Kit;Human glutamate receptor subunit 3 ELISA Kit;Human glutamate receptor, ionotrophic, AMPA 3 ELISA Kit;Human glutamate receptor, ionotropic, AMPA 3 ELISA Kit;

  • Catalog
    E016736
  • species
    Human
  • GeneGRIA3
  • Standard CurveHuman GRIA3 ELISA Kit
  • Other Species Mouse Gria3 ELISA KitMonkey GRIA3 ELISA KitRat Gria3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GRIA3. No significant cross-reactivity or interference between GRIA3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GRIA3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman GRIA3 ELISA Kit allows for the in vitro quantitative determination of GRIA3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Intended Uses: This GRIA3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GRIA3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||GRIA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRIA3 antibody and an GRIA3-HRP conjugate. The assay sample and buffer are incubated together with GRIA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRIA3 concentration since GRIA3 from samples and GRIA3-HRP conjugate compete for the anti-GRIA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GRIA3 from the sample, fewer sites are left to bind GRIA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRIA3 concentration in each sample is interpolated from this standard curve.
  • Human Glutamate receptor 3 Protein information
  • Uniprot ID GRIA3_HUMAN
  • Uniprot AC P42263; D3DTF1; Q4VXD5; Q4VXD6; Q9HDA0; Q9HDA1; Q9HDA2; Q9P0H1;
  • UniGene Hs.377070;
  • GeneID 2892
  • KEGG hsa:2892;
  • Human Glutamate receptor 3 Protein SEQUENCE
  • SEQUENCE 894 AA; 101157 MW; 178589A870E0D102 CRC64;

    MARQKKMGQS VLRAVFFLVL GLLGHSHGGF PNTISIGGLF MRNTVQEHSA

    FRFAVQLYNT NQNTTEKPFH LNYHVDHLDS SNSFSVTNAF CSQFSRGVYA

    IFGFYDQMSM NTLTSFCGAL HTSFVTPSFP TDADVQFVIQ MRPALKGAIL

    SLLGHYKWEK FVYLYDTERG FSILQAIMEA AVQNNWQVTA RSVGNIKDVQ

    EFRRIIEEMD RRQEKRYLID CEVERINTIL EQVVILGKHS RGYHYMLANL

    GFTDILLERV MHGGANITGF QIVNNENPMV QQFIQRWVRL DEREFPEAKN

    APLKYTSALT HDAILVIAEA FRYLRRQRVD VSRRGSAGDC LANPAVPWSQ

    GIDIERALKM VQVQGMTGNI QFDTYGRRTN YTIDVYEMKV SGSRKAGYWN

    EYERFVPFSD QQISNDSASS ENRTIVVTTI LESPYVMYKK NHEQLEGNER

    YEGYCVDLAY EIAKHVRIKY KLSIVGDGKY GARDPETKIW NGMVGELVYG

    RADIAVAPLT ITLVREEVID FSKPFMSLGI SIMIKKPQKS KPGVFSFLDP

    LAYEIWMCIV FAYIGVSVVL FLVSRFSPYE WHLEDNNEEP RDPQSPPDPP

    NEFGIFNSLW FSLGAFMQQG CDISPRSLSG RIVGGVWWFF TLIIISSYTA

    NLAAFLTVER MVSPIESAED LAKQTEIAYG TLDSGSTKEF FRRSKIAVYE

    KMWSYMKSAE PSVFTKTTAD GVARVRKSKG KFAFLLESTM NEYIEQRKPC

    DTMKVGGNLD SKGYGVATPK GSALRNAVNL AVLKLNEQGL LDKLKNKWWY

    DKGECGSGGG DSKDKTSALS LSNVAGVFYI LVGGLGLAMM VALIEFCYKS

    RAESKRMKLT KNTQNFKPAP ATNTQNYATY REGYNVYGTE SVKI

  • UCSC uc033etl.2; human. [P42263-1];



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