Human AMACR ELISA Kit

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  • Alternative name

    Human 2-methylacyl-CoA racemase ELISA Kit;Human AMACRD ELISA Kit;Human CBAS4 ELISA Kit;Human P504S ELISA Kit;Human RACE ELISA Kit;Human RM ELISA Kit;Human alpha-methylacyl-CoA racemase ELISA Kit;

  • Catalog
    E001671
  • species
    Human
  • GeneAMACR
  • Standard CurveHuman AMACR ELISA Kit
  • Other Species Mouse Amacr ELISA KitRat Amacr ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Alpha-Methylacyl Coenzyme A Racemase (aMACR). No significant cross-reactivity or interference between Alpha-Methylacyl Coenzyme A Racemase (aMACR) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.066ng/mL.
  • Intended UseHuman AMACR ELISA Kit allows for the in vitro quantitative determination of AMACR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Methylacyl Coenzyme A Racemase (aMACR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Methylacyl Coenzyme A Racemase (aMACR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Methylacyl Coenzyme A Racemase (aMACR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Methylacyl Coenzyme A Racemase (aMACR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Alpha-methylacyl-CoA racemase Protein information
  • Uniprot ID AMACR_HUMAN
  • Uniprot AC Q9Y3Q1;
  • UniGene Hs.508343;
  • GeneID 23600
  • KEGG hsa:23600;
  • Human Alpha-methylacyl-CoA racemase Protein SEQUENCE
  • SEQUENCE 382 AA; 42387 MW; E967D3221A90BEF8 CRC64;

    MALQGISVVE LSGLAPGPFC AMVLADFGAR VVRVDRPGSR YDVSRLGRGK

    RSLVLDLKQP RGAAVLRRLC KRSDVLLEPF RRGVMEKLQL GPEILQRENP

    RLIYARLSGF GQSGSFCRLA GHDINYLALS GVLSKIGRSG ENPYAPLNLL

    ADFAGGGLMC ALGIIMALFD RTRTGKGQVI DANMVEGTAY LSSFLWKTQK

    LSLWEAPRGQ NMLDGGAPFY TTYRTADGEF MAVGAIEPQF YELLIKGLGL

    KSDELPNQMS MDDWPEMKKK FADVFAEKTK AEWCQIFDGT DACVTPVLTF

    EEVVHHDHNK ERGSFITSEE QDVSPRPAPL LLNTPAIPSF KRDPFIGEHT

    EEILEEFGFS REEIYQLNSD KIIESNKVKA SL

  • UCSC uc003jig.4; human. [Q9UHK6-1];


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