Human AMACR ELISA Kit

Alternative name
Human 2-methylacyl-CoA racemase ELISA Kit;Human AMACRD ELISA Kit;Human CBAS4 ELISA Kit;Human P504S ELISA Kit;Human RACE ELISA Kit;Human RM ELISA Kit;Human alpha-methylacyl-CoA racemase ELISA Kit;
Catalog
E001671
species
Human
GeneAMACR
Standard Curve
Other Species Mouse Amacr ELISA Kit Rat Amacr ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Alpha-Methylacyl Coenzyme A Racemase (aMACR). No significant cross-reactivity or interference between Alpha-Methylacyl Coenzyme A Racemase (aMACR) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.066ng/mL.
Intended UseHuman AMACR ELISA Kit allows for the in vitro quantitative determination of AMACR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Methylacyl Coenzyme A Racemase (aMACR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Methylacyl Coenzyme A Racemase (aMACR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Methylacyl Coenzyme A Racemase (aMACR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Methylacyl Coenzyme A Racemase (aMACR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Alpha-methylacyl-CoA racemase Protein information
Uniprot ID AMACR_HUMAN
Uniprot AC Q9Y3Q1;
UniGene Hs.508343;
GeneID 23600
KEGG hsa:23600;
Human Alpha-methylacyl-CoA racemase Protein SEQUENCE
SEQUENCE 382 AA; 42387 MW; E967D3221A90BEF8 CRC64;
MALQGISVVE LSGLAPGPFC AMVLADFGAR VVRVDRPGSR YDVSRLGRGK
RSLVLDLKQP RGAAVLRRLC KRSDVLLEPF RRGVMEKLQL GPEILQRENP
RLIYARLSGF GQSGSFCRLA GHDINYLALS GVLSKIGRSG ENPYAPLNLL
ADFAGGGLMC ALGIIMALFD RTRTGKGQVI DANMVEGTAY LSSFLWKTQK
LSLWEAPRGQ NMLDGGAPFY TTYRTADGEF MAVGAIEPQF YELLIKGLGL
KSDELPNQMS MDDWPEMKKK FADVFAEKTK AEWCQIFDGT DACVTPVLTF
EEVVHHDHNK ERGSFITSEE QDVSPRPAPL LLNTPAIPSF KRDPFIGEHT
EEILEEFGFS REEIYQLNSD KIIESNKVKA SL
UCSC uc003jig.4; human. [Q9UHK6-1];

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Human AMACR ELISA Kit

Human 2-methylacyl-CoA racemase ELISA Kit;Human AMACRD ELISA Kit;Human CBAS4 ELISA Kit;Human P504S ELISA Kit;Human RACE ELISA Kit;Human RM ELISA Kit;Human alpha-methylacyl-CoA racemase ELISA Kit;