Human GCLM ELISA Kit

Alternative name
Human GCS light chain ELISA Kit;Human gamma-ECS regulatory subunit ELISA Kit;Human gamma-glutamylcysteine synthetase regulatory subunit ELISA Kit;Human Glutamate--cysteine ligase modifier subunit ELISA Kit;Human GLCLR ELISA Kit;Human glutamate-cysteine ligase modifier subunit ELISA Kit;Human glutamate--cysteine ligase regulatory subunit ELISA Kit;Human GSC light chain ELISA Kit;Human glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD) ELISA Kit;Human glutamate-cysteine ligase modifier subunit delta2 alternative splicing ELISA Kit;Human glutamate-cysteine ligase regulatory protein ELISA Kit;
Catalog
E016698
species
Human
GeneGCLM
Standard Curve
Other Species Mouse Gclm ELISA Kit Bovine GCLM ELISA Kit Rat Gclm ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Glutamate Cysteine Ligase, Modifier Subunit (GCLM). No significant cross-reactivity or interference between Glutamate Cysteine Ligase, Modifier Subunit (GCLM) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.061ng/mL.
Intended UseHuman GCLM ELISA Kit allows for the in vitro quantitative determination of GCLM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Cysteine Ligase, Modifier Subunit (GCLM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Cysteine Ligase, Modifier Subunit (GCLM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Cysteine Ligase, Modifier Subunit (GCLM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Cysteine Ligase, Modifier Subunit (GCLM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Glutamate--cysteine ligase regulatory subunit Protein information
Uniprot ID GSH0_HUMAN
Uniprot AC P48507; A8K334; D3DT45; M5A959; Q6FHC1; Q9NPX9; Q9NU74;
UniGene Hs.315562; Hs.596052; Hs.711318;
GeneID 2730
KEGG hsa:2730;
Human Glutamate--cysteine ligase regulatory subunit Protein SEQUENCE
SEQUENCE 274 AA; 30727 MW; 88F73E22322E40B2 CRC64;
MGTDSRAAKA LLARARTLHL QTGNLLNWGR LRKKCPSTHS EELHDCIQKT
LNEWSSQINP DLVREFPDVL ECTVSHAVEK INPDEREEMK VSAKLFIVES
NSSSSTRSAV DMACSVLGVA QLDSVIIASP PIEDGVNLSL EHLQPYWEEL
ENLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL
TAFAKQFDIQ LLTHNDPKEL LSEASFQEAL QESIPDIQAH EWVPLWLLRY
SVIVKSRGII KSKGYILQAK RRGS
UCSC uc001dqg.2; human. [P48507-1];

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Human GCLM ELISA Kit