Human GUSB ELISA Kit

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  • Alternative name

    Human beta-G1 ELISA Kit;Human BG ELISA Kit;Human MPS7 ELISA Kit;Human glucuronidase beta ELISA Kit;Human beta-glucuronidase ELISA Kit;Human beta-D-glucuronidase ELISA Kit;

  • Catalog
    E016680
  • species
    Human
  • GeneGUSB
  • Standard CurveHuman GUSB ELISA Kit
  • Other Species Mouse Gusb ELISA KitCanine GUSB ELISA KitPorcine GUSB ELISA KitRat Gusb ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Glucuronidase Beta (GUSb). No significant cross-reactivity or interference between Glucuronidase Beta (GUSb) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.074ng/mL.
  • Intended UseHuman GUSB ELISA Kit allows for the in vitro quantitative determination of GUSB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucuronidase Beta (GUSb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucuronidase Beta (GUSb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucuronidase Beta (GUSb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucuronidase Beta (GUSb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Beta-glucuronidase Protein information
  • Uniprot ID BGLR_HUMAN
  • Uniprot AC P08236; B4E1F6; E9PCV0; Q549U0; Q96CL9;
  • UniGene Hs.255230;
  • GeneID 2990
  • KEGG hsa:2990;
  • Human Beta-glucuronidase Protein SEQUENCE
  • SEQUENCE 651 AA; 74732 MW; 6BA7B1D935C9ABBD CRC64;

    MARGSAVAWA ALGPLLWGCA LGLQGGMLYP QESPSRECKE LDGLWSFRAD

    FSDNRRRGFE EQWYRRPLWE SGPTVDMPVP SSFNDISQDW RLRHFVGWVW

    YEREVILPER WTQDLRTRVV LRIGSAHSYA IVWVNGVDTL EHEGGYLPFE

    ADISNLVQVG PLPSRLRITI AINNTLTPTT LPPGTIQYLT DTSKYPKGYF

    VQNTYFDFFN YAGLQRSVLL YTTPTTYIDD ITVTTSVEQD SGLVNYQISV

    KGSNLFKLEV RLLDAENKVV ANGTGTQGQL KVPGVSLWWP YLMHERPAYL

    YSLEVQLTAQ TSLGPVSDFY TLPVGIRTVA VTKSQFLING KPFYFHGVNK

    HEDADIRGKG FDWPLLVKDF NLLRWLGANA FRTSHYPYAE EVMQMCDRYG

    IVVIDECPGV GLALPQFFNN VSLHHHMQVM EEVVRRDKNH PAVVMWSVAN

    EPASHLESAG YYLKMVIAHT KSLDPSRPVT FVSNSNYAAD KGAPYVDVIC

    LNSYYSWYHD YGHLELIQLQ LATQFENWYK KYQKPIIQSE YGAETIAGFH

    QDPPLMFTEE YQKSLLEQYH LGLDQKRRKY VVGELIWNFA DFMTEQSPTR

    VLGNKKGIFT RQRQPKSAAF LLRERYWKIA NETRYPHSVA KSQCLENSLF

    T

  • UCSC uc003tun.4; human. [P08236-1];



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