-
Alternative name
78 kDa glucose-regulated protein ELISA KIT; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 ELISA KIT; Heat shock 70 kDa protein 5 ELISA KIT; Immunoglobulin heavy chain-binding protein ELISA KIT; BiPHSPA5 ELISA KIT; GRP78 ELISA KIT; GRP-78 ELISA KIT; BiP ELISA KIT
-
Catalog
E016611
-
species
Human
-
GeneGRP78
-
Standard Curve
-
Other Species
Mouse GRP78 ELISA Kit
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of GRP78. No significant cross-reactivity or interference between GRP78 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GRP78 and all the analogues, therefore, cross reaction may still exist in some cases.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity0.1 ng/mL.
-
Intended UseHuman GRP78 ELISA Kit allows for the in vitro quantitative determination of GRP78 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalIntended Uses: This GRP78 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GRP78. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||GRP78 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRP78 antibody and an GRP78-HRP conjugate. The assay sample and buffer are incubated together with GRP78-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRP78 concentration since GRP78 from samples and GRP78-HRP conjugate compete for the anti-GRP78 antibody binding site. Since the number of sites is limited, as more sites are occupied by GRP78 from the sample, fewer sites are left to bind GRP78-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRP78 concentration in each sample is interpolated from this standard curve.
-