Human G6PD ELISA Kit

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  • Alternative name

    Human G6PD ELISA Kit;Human G6PD1 ELISA Kit;Human glucose-6-phosphate dehydrogenase ELISA Kit;Human glucose-6-phosphate 1-dehydrogenase ELISA Kit;

  • Catalog
    E016597
  • species
    Human
  • GeneG6PD
  • Standard CurveHuman G6PD ELISA Kit
  • Other Species Mouse G6PD ELISA KitHamster G6PD ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Glucose 6 Phosphate Dehydrogenase (G6PD). No significant cross-reactivity or interference between Glucose 6 Phosphate Dehydrogenase (G6PD) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.274ng/mL.
  • Intended UseHuman G6PD ELISA Kit allows for the in vitro quantitative determination of G6PD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose 6 Phosphate Dehydrogenase (G6PD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose 6 Phosphate Dehydrogenase (G6PD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose 6 Phosphate Dehydrogenase (G6PD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose 6 Phosphate Dehydrogenase (G6PD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Glucose-6-phosphate 1-dehydrogenase Protein information
  • Uniprot ID G6PD_HUMAN
  • Uniprot AC P11413; D3DWX9; Q16000; Q16765; Q8IU70; Q8IU88; Q8IUA6; Q96PQ2;
  • UniGene Hs.461047; Hs.684904;
  • GeneID 2539
  • KEGG hsa:2539;
  • Human Glucose-6-phosphate 1-dehydrogenase Protein SEQUENCE
  • SEQUENCE 515 AA; 59257 MW; F2B775340640A96F CRC64;

    MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP

    TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED

    FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV

    TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID

    HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF

    DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA

    NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD

    GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV

    YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM

    HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG

    FQYEGTYKWV NPHKL

  • UCSC uc004flx.3; human. [P11413-1];



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