Human AHSP ELISA Kit

Alternative name
Human erythroid differentiation-related factor ELISA Kit;Human Erythroid-associated factor ELISA Kit;Human EDRF ELISA Kit;Human ERAF ELISA Kit;Human alpha hemoglobin stabilizing protein ELISA Kit;Human alpha-hemoglobin-stabilizing protein ELISA Kit;Human alpha hemoglobin stabilising protein ELISA Kit;Human erythroid differentiation associated factor ELISA Kit;
Catalog
E001659
species
Human
GeneAHSP
SpecificityThis assay has high sensitivity and excellent specificity for detection of Alpha-Hemoglobin Stabilizing Protein (aHSP). No significant cross-reactivity or interference between Alpha-Hemoglobin Stabilizing Protein (aHSP) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.055ng/mL.
Intended UseHuman AHSP ELISA Kit allows for the in vitro quantitative determination of AHSP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Hemoglobin Stabilizing Protein (aHSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Hemoglobin Stabilizing Protein (aHSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Hemoglobin Stabilizing Protein (aHSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Hemoglobin Stabilizing Protein (aHSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Alpha-hemoglobin-stabilizing protein Protein information
Uniprot ID AHSP_HUMAN
Uniprot AC Q9NZD4; Q8TD01;
UniGene Hs.274309;
GeneID 51327
KEGG hsa:51327;
Human Alpha-hemoglobin-stabilizing protein Protein SEQUENCE
SEQUENCE 102 AA; 11840 MW; 275DDF8BC670EF20 CRC64;
MALLKANKDL ISAGLKEFSV LLNQQVFNDP LVSEEDMVTV VEDWMNFYIN
YYRQQVTGEP QERDKALQEL RQELNTLANP FLAKYRDFLK SHELPSHPPP
SS
UCSC uc002ecj.4; human.;

Human AHSP ELISA Kit

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