Human GRb ELISA Kit

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  • Alternative name

    Glucocorticoid receptor ELISA KIT; Nuclear receptor subfamily 3 group C member 1NR3C1 ELISA KIT; GRL ELISA KIT; GR ELISA KIT

  • Catalog
    E016557
  • species
    Human
  • GeneGRb
  • Standard CurveHuman GRb ELISA Kit
  • Other Species Mouse GRb ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Glucocorticoid Receptor Beta (GRb). No significant cross-reactivity or interference between Glucocorticoid Receptor Beta (GRb) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.114ng/mL.
  • Intended UseHuman GRb ELISA Kit allows for the in vitro quantitative determination of GRb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucocorticoid Receptor Beta (GRb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucocorticoid Receptor Beta (GRb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucocorticoid Receptor Beta (GRb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucocorticoid Receptor Beta (GRb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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