Human GITR ELISA Kit

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  • Alternative name

    Tumor necrosis factor receptor superfamily member 18 ELISA KIT; Glucocorticoid-induced TNFR-related protein ELISA KIT; CD_antigen: CD357Tnfrsf18 ELISA KIT; Gitr ELISA KIT

  • Catalog
    E016545
  • species
    Human
  • GeneGITR
  • Standard CurveHuman GITR ELISA Kit
  • Other Species Mouse GITR ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR). No significant cross-reactivity or interference between Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 5.9pg/mL.
  • Intended UseHuman GITR ELISA Kit allows for the in vitro quantitative determination of GITR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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