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  • Alternative name

    Glia-derived nexin ELISA KIT; Peptidase inhibitor 7 ELISA KIT; PI-7 ELISA KIT; Protease nexin 1 ELISA KIT; PN-1 ELISA KIT; Protease nexin I ELISA KIT; Serpin E2SERPINE2 ELISA KIT; PI7 ELISA KIT; PN1 ELISA KIT; GDN ELISA KIT; PI-7 ELISA KIT; PN-1 ELISA KIT

  • Catalog
  • species
  • GeneGDN
  • Standard CurveHuman GDN ELISA Kit
  • Other Species Mouse GDN ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Glia Derived Nexin (GDN). No significant cross-reactivity or interference between Glia Derived Nexin (GDN) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.135ng/mL.
  • Intended UseHuman GDN ELISA Kit allows for the in vitro quantitative determination of GDN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glia Derived Nexin (GDN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glia Derived Nexin (GDN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glia Derived Nexin (GDN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glia Derived Nexin (GDN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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