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Alternative name
Glucokinase regulatory protein ELISA KIT; GCKR ELISA KIT; GKRP ELISA KIT; Glucokinase regulator ELISA KIT
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Catalog
E016432
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species
Human
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GeneGKRP
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SpecificityThis kit recognizes natural and recombinant Human GKRP. No significant cross-reactivity or interference between Human GKRP and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.375ng/mL
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Intended UseHuman GKRP ELISA Kit allows for the in vitro quantitative determination of GKRP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GKRP concentrations in serum, plasma and other biological fluids.
Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GKRP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GKRP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GKRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GKRP. You can calculate the concentration of GKRP in the samples by comparing the OD of the samples to the standard curve.
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