Human GDNF ELISA Kit

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  • Alternative name

    Human hGDNF ELISA Kit;Human astrocyte-derived trophic factor ELISA Kit;Human ATF ELISA Kit;Human ATF1 ELISA Kit;Human ATF2 ELISA Kit;Human HFB1-GDNF ELISA Kit;Human HSCR3 ELISA Kit;Human glial cell derived neurotrophic factor ELISA Kit;Human glial cell line-derived neurotrophic factor ELISA Kit;

  • Catalog
    E016301
  • species
    Human
  • GeneGDNF
  • Standard CurveHuman GDNF ELISA Kit
  • Other Species Mouse Gdnf ELISA KitRat Gdnf ELISA Kit
  • SpecificityNatural and recombinant human GDNF
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 4pg/ml
  • Intended UseHuman GDNF ELISA Kit allows for the in vitro quantitative determination of GDNF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human GDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDNF has been precoated onto 96-well plates. Standards (NSO,R109-I211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GDNF amount of sample captured in plate. Background: Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor thatpromotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, inaddition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility inthe treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.1 GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamineuptake in midbrain cultures.2
  • Human Glial cell line-derived neurotrophic factor Protein information
  • Uniprot ID GDNF_HUMAN
  • Uniprot AC Q9UD33; Q9UMV2; Q9UP67; Q9UP97;
  • UniGene Hs.248114;
  • GeneID 2668
  • KEGG hsa:2668;
  • Human Glial cell line-derived neurotrophic factor Protein SEQUENCE
  • SEQUENCE 211 AA; 23720 MW; A0D1EBF77FC82691 CRC64;

    MKLWDVVAVC LVLLHTASAF PLPAGKRPPE APAEDRSLGR RRAPFALSSD

    SNMPEDYPDQ FDDVMDFIQA TIKRLKRSPD KQMAVLPRRE RNRQAAAANP

    ENSRGKGRRG QRGKNRGCVL TAIHLNVTDL GLGYETKEEL IFRYCSGSCD

    AAETTYDKIL KNLSRNRRLV SDKVGQACCR PIAFDDDLSF LDDNLVYHIL

    RKHSAKRCGC I

  • UCSC uc011cpd.2; human. [P39905-1];



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