Human GDF1 ELISA Kit

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  • Alternative name

    Human GDF-1 ELISA Kit;Human DORV ELISA Kit;Human DTGA3 ELISA Kit;Human RAI ELISA Kit;Human growth differentiation factor 1 ELISA Kit;Human embryonic growth/differentiation factor 1 ELISA Kit;

  • Catalog
    E016285
  • species
    Human
  • GeneGDF1
  • Other Species Mouse Gdf1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human GDF1. No significant cross-reactivity or interference between Human GDF1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human GDF1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity18.75pg/mL
  • Intended UseHuman GDF1 ELISA Kit allows for the in vitro quantitative determination of GDF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GDF1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GDF1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human GDF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GDF1. You can calculate the concentration of Human GDF1 in the samples by comparing the OD of the samples to the standard curve.
  • Human Embryonic growth/differentiation factor 1 Protein information
  • Uniprot ID GDF1_HUMAN
  • Uniprot AC P27539; O43344;
  • UniGene Hs.412355;
  • GeneID 2657
  • KEGG hsa:2657;
  • Human Embryonic growth/differentiation factor 1 Protein SEQUENCE
  • SEQUENCE 372 AA; 39475 MW; 10A4893F063047B9 CRC64;

    MPPPQQGPCG HHLLLLLALL LPSLPLTRAP VPPGPAAALL QALGLRDEPQ

    GAPRLRPVPP VMWRLFRRRD PQETRSGSRR TSPGVTLQPC HVEELGVAGN

    IVRHIPDRGA PTRASEPASA AGHCPEWTVV FDLSAVEPAE RPSRARLELR

    FAAAAAAAPE GGWELSVAQA GQGAGADPGP VLLRQLVPAL GPPVRAELLG

    AAWARNASWP RSLRLALALR PRAPAACARL AEASLLLVTL DPRLCHPLAR

    PRRDAEPVLG GGPGGACRAR RLYVSFREVG WHRWVIAPRG FLANYCQGQC

    ALPVALSGSG GPPALNHAVL RALMHAAAPG AADLPCCVPA RLSPISVLFF

    DNSDNVVLRQ YEDMVVDECG CR

  • UCSC uc060vuk.1; human.;



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