Human GATA5 ELISA Kit

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  • Alternative name

    Human GATA-binding factor 5 ELISA Kit;Human GATAS ELISA Kit;Human bB379O24.1 ELISA Kit;Human GATA binding protein 5 ELISA Kit;Human transcription factor GATA-5 ELISA Kit;Human GATA binding factor-5 ELISA Kit;

  • Catalog
    E016258
  • species
    Human
  • GeneGATA5
  • Standard CurveHuman GATA5 ELISA Kit
  • Other Species Mouse Gata5 ELISA KitBovine GATA5 ELISA KitChicken GATA5 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman GATA5 ELISA Kit allows for the in vitro quantitative determination of GATA5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: GATA5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GATA5. Standards or samples are then added to the microtiter plate wells and GATA5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GATA5 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for GATA5 are added to each well to "sandwich" the GATA5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GATA5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GATA5 concentration in each sample is interpolated from this standard curve.
  • Human Transcription factor GATA-5 Protein information
  • Uniprot ID GATA5_HUMAN
  • Uniprot AC Q9BWX5; D9ZGF7; Q17RE2; Q86VU4;
  • UniGene Hs.352250;
  • GeneID 140628
  • KEGG hsa:140628;
  • Human Transcription factor GATA-5 Protein SEQUENCE
  • SEQUENCE 397 AA; 41299 MW; 5DFBA02085695C57 CRC64;

    MYQSLALAAS PRQAAYADSG SFLHAPGAGS PMFVPPARVP SMLSYLSGCE

    PSPQPPELAA RPGWAQTATA DSSAFGPGSP HPPAAHPPGA TAFPFAHSPS

    GPGSGGSAGG RDGSAYQGAL LPREQFAAPL GRPVGTSYSA TYPAYVSPDV

    AQSWTAGPFD GSVLHGLPGR RPTFVSDFLE EFPGEGRECV NCGALSTPLW

    RRDGTGHYLC NACGLYHKMN GVNRPLVRPQ KRLSSSRRAG LCCTNCHTTN

    TTLWRRNSEG EPVCNACGLY MKLHGVPRPL AMKKESIQTR KRKPKTIAKA

    RGSSGSTRNA SASPSAVAST DSSAATSKAK PSLASPVCPG PSMAPQASGQ

    EDDSLAPGHL EFKFEPEDFA FPSTAPSPQA GLRGALRQEA WCALALA

  • UCSC uc002ycx.1; human.;


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