Human GATA3 ELISA Kit

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  • Alternative name

    Human GATA-binding factor 3 ELISA Kit;Human HDR ELISA Kit;Human HDRS ELISA Kit;Human GATA binding protein 3 ELISA Kit;Human trans-acting T-cell-specific transcription factor GATA-3 ELISA Kit;

  • Catalog
    E016253
  • species
    Human
  • GeneGATA3
  • Standard CurveHuman GATA3 ELISA Kit
  • Other Species Mouse Gata3 ELISA KitBovine GATA3 ELISA KitChicken GATA3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GATA Binding Protein 3 (GATA3). No significant cross-reactivity or interference between GATA Binding Protein 3 (GATA3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 11.4pg/mL.
  • Intended UseHuman GATA3 ELISA Kit allows for the in vitro quantitative determination of GATA3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GATA Binding Protein 3 (GATA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GATA Binding Protein 3 (GATA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GATA Binding Protein 3 (GATA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GATA Binding Protein 3 (GATA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Trans-acting T-cell-specific transcription factor GATA-3 Protein information
  • Uniprot ID GATA3_HUMAN
  • Uniprot AC P23771; Q5VWG7; Q5VWG8; Q96J16;
  • UniGene Hs.524134;
  • GeneID 2625
  • KEGG hsa:2625;
  • Human Trans-acting T-cell-specific transcription factor GATA-3 Protein SEQUENCE
  • SEQUENCE 443 AA; 47916 MW; F24C58681E1D02A5 CRC64;

    MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LSHSYMDAAQ YPLPEEVDVL

    FNIDGQGNHV PPYYGNSVRA TVQRYPPTHH GSQVCRPPLL HGSLPWLDGG

    KALGSHHTAS PWNLSPFSKT SIHHGSPGPL SVYPPASSSS LSGGHASPHL

    FTFPPTPPKD VSPDPSLSTP GSAGSARQDE KECLKYQVPL PDSMKLESSH

    SRGSMTALGG ASSSTHHPIT TYPPYVPEYS SGLFPPSSLL GGSPTGFGCK

    SRPKARSSTG RECVNCGATS TPLWRRDGTG HYLCNACGLY HKMNGQNRPL

    IKPKRRLSAA RRAGTSCANC QTTTTTLWRR NANGDPVCNA CGLYYKLHNI

    NRPLTMKKEG IQTRNRKMSS KSKKCKKVHD SLEDFPKNSS FNPAALSRHM

    SSLSHISPFS HSSHMLTTPT PMHPPSSLSF GPHHPSSMVT AMG

  • UCSC uc001ijz.4; human. [P23771-1];




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