Human GCA ELISA Kit

Alternative name
Human GCL ELISA Kit;Human grancalcin ELISA Kit;Human grancalcin, EF-hand calcium-binding protein ELISA Kit;Human grancalcin, penta-EF-hand protein ELISA Kit;
Catalog
E016212
species
Human
GeneGCA
Other Species Mouse Gca ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman GCA ELISA Kit allows for the in vitro quantitative determination of GCA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalPrinciple of the Assay: GCA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GCA. Standards or samples are then added to the microtiter plate wells and GCA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GCA present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for GCA are added to each well to "sandwich" the GCA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GCA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCA concentration in each sample is interpolated from this standard curve.
Human Grancalcin Protein information
Uniprot ID GRAN_HUMAN
Uniprot AC P28676; B2R5X3; Q53TB5; Q59EP3;
UniGene Hs.377894;
GeneID 25801
KEGG hsa:25801;
Human Grancalcin Protein SEQUENCE
SEQUENCE 217 AA; 24010 MW; 88CA4DDF835AFFE4 CRC64;
MAYPGYGGGF GNFSIQVPGM QMGQPVPETG PAILLDGYSG PAYSDTYSSA
GDSVYTYFSA VAGQDGEVDA EELQRCLTQS GINGTYSPFS LETCRIMIAM
LDRDHTGKMG FNAFKELWAA LNAWKENFMT VDQDGSGTVE HHELRQAIGL
MGYRLSPQTL TTIVKRYSKN GRIFFDDYVA CCVKLRALTD FFRKRDHLQQ
GSANFIYDDF LQGTMAI
UCSC uc002ucg.4; human.;

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Human GCA ELISA Kit

Human GCL ELISA Kit;Human grancalcin ELISA Kit;Human grancalcin, EF-hand calcium-binding protein ELISA Kit;Human grancalcin, penta-EF-hand protein ELISA Kit;