Human PGC ELISA Kit

Alternative name
Human pepsinogen C ELISA Kit;Human PEPC ELISA Kit;Human PGII ELISA Kit;Human progastricsin ELISA Kit;Human gastricsin ELISA Kit;Human pepsin C ELISA Kit;Human pepsinogen group II ELISA Kit;Human preprogastricsin ELISA Kit;
Catalog
E016201
species
Human
GenePGC
Standard Curve
Other Species Mouse Pgc ELISA Kit Guinea Pig PGC ELISA Kit Porcine PGC ELISA Kit Rabbit PGC ELISA Kit Rat Pgc ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of PGC. No significant cross-reactivity or interference between PGC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PGC and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman PGC ELISA Kit allows for the in vitro quantitative determination of PGC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This PGC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PGC. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||PGC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGC antibody and an PGC-HRP conjugate. The assay sample and buffer are incubated together with PGC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGC concentration since PGC from samples and PGC-HRP conjugate compete for the anti-PGC antibody binding site. Since the number of sites is limited, as more sites are occupied by PGC from the sample, fewer sites are left to bind PGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGC concentration in each sample is interpolated from this standard curve.
Human Gastricsin Protein information
Uniprot ID PEPC_HUMAN
Uniprot AC P20142; B4DVZ3; Q5T3D7; Q5T3D8;
UniGene Hs.1867;
GeneID 5225
KEGG hsa:5225;
Human Gastricsin Protein SEQUENCE
SEQUENCE 388 AA; 42426 MW; F862DFDC1438BB92 CRC64;
MKWMVVVLVC LQLLEAAVVK VPLKKFKSIR ETMKEKGLLG EFLRTHKYDP
AWKYRFGDLS VTYEPMAYMD AAYFGEISIG TPPQNFLVLF DTGSSNLWVP
SVYCQSQACT SHSRFNPSES STYSTNGQTF SLQYGSGSLT GFFGYDTLTV
QSIQVPNQEF GLSENEPGTN FVYAQFDGIM GLAYPALSVD EATTAMQGMV
QEGALTSPVF SVYLSNQQGS SGGAVVFGGV DSSLYTGQIY WAPVTQELYW
QIGIEEFLIG GQASGWCSEG CQAIVDTGTS LLTVPQQYMS ALLQATGAQE
DEYGQFLVNC NSIQNLPSLT FIINGVEFPL PPSSYILSNN GYCTVGVEPT
YLSSQNGQPL WILGDVFLRS YYSVYDLGNN RVGFATAA
UCSC uc003ora.3; human. [P20142-1];

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Category

Human PGC ELISA Kit

Human pepsinogen C ELISA Kit;Human PEPC ELISA Kit;Human PGII ELISA Kit;Human progastricsin ELISA Kit;Human gastricsin ELISA Kit;Human pepsin C ELISA Kit;Human pepsinogen group II ELISA Kit;Human preprogastricsin ELISA Kit;