Human GIF ELISA Kit

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  • Alternative name

    Human intrinsic factor ELISA Kit;Human IF ELISA Kit;Human INF ELISA Kit;Human IFMH ELISA Kit;Human TCN3 ELISA Kit;Human gastric intrinsic factor ELISA Kit;Human gastric intrinsic factor (vitamin B synthesis) ELISA Kit;

  • Catalog
    E016194
  • species
    Human
  • GeneGIF
  • Standard CurveHuman GIF ELISA Kit
  • Other Species Mouse Gif ELISA KitCanine GIF ELISA KitRat Gif ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Gastric Intrinsic Factor (GIF). No significant cross-reactivity or interference between Gastric Intrinsic Factor (GIF) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.95ng/mL.
  • Intended UseHuman GIF ELISA Kit allows for the in vitro quantitative determination of GIF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gastric Intrinsic Factor (GIF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gastric Intrinsic Factor (GIF) and unlabeled Gastric Intrinsic Factor (GIF) (Standards or samples) with the pre-coated antibody specific to Gastric Intrinsic Factor (GIF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gastric Intrinsic Factor (GIF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gastric Intrinsic Factor (GIF) in the sample.
  • Human Gastric intrinsic factor Protein information
  • Uniprot ID IF_HUMAN
  • Uniprot AC P27352; B2RAN8; B4DVZ1;
  • UniGene Hs.110014;
  • GeneID 2694
  • KEGG hsa:2694;
  • Human Gastric intrinsic factor Protein SEQUENCE
  • SEQUENCE 417 AA; 45416 MW; F8B376E03A3D0F3C CRC64;

    MAWFALYLLS LLWATAGTST QTQSSCSVPS AQEPLVNGIQ VLMENSVTSS

    AYPNPSILIA MNLAGAYNLK AQKLLTYQLM SSDNNDLTIG QLGLTIMALT

    SSCRDPGDKV SILQRQMENW APSSPNAEAS AFYGPSLAIL ALCQKNSEAT

    LPIAVRFAKT LLANSSPFNV DTGAMATLAL TCMYNKIPVG SEEGYRSLFG

    QVLKDIVEKI SMKIKDNGII GDIYSTGLAM QALSVTPEPS KKEWNCKKTT

    DMILNEIKQG KFHNPMSIAQ ILPSLKGKTY LDVPQVTCSP DHEVQPTLPS

    NPGPGPTSAS NITVIYTINN QLRGVELLFN ETINVSVKSG SVLLVVLEEA

    QRKNPMFKFE TTMTSWGLVV SSINNIAENV NHKTYWQFLS GVTPLNEGVA

    DYIPFNHEHI TANFTQY

  • UCSC uc001noi.4; human. [P27352-1];


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