Human GIF ELISA Kit

Alternative name
Human intrinsic factor ELISA Kit;Human IF ELISA Kit;Human INF ELISA Kit;Human IFMH ELISA Kit;Human TCN3 ELISA Kit;Human gastric intrinsic factor ELISA Kit;Human gastric intrinsic factor (vitamin B synthesis) ELISA Kit;
Catalog
E016194
species
Human
GeneGIF
SpecificityThis assay has high sensitivity and excellent specificity for detection of Gastric Intrinsic Factor (GIF). No significant cross-reactivity or interference between Gastric Intrinsic Factor (GIF) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.95ng/mL.
Intended UseHuman GIF ELISA Kit allows for the in vitro quantitative determination of GIF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gastric Intrinsic Factor (GIF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gastric Intrinsic Factor (GIF) and unlabeled Gastric Intrinsic Factor (GIF) (Standards or samples) with the pre-coated antibody specific to Gastric Intrinsic Factor (GIF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gastric Intrinsic Factor (GIF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gastric Intrinsic Factor (GIF) in the sample.
Human Gastric intrinsic factor Protein information
Uniprot ID IF_HUMAN
Uniprot AC P27352; B2RAN8; B4DVZ1;
UniGene Hs.110014;
GeneID 2694
KEGG hsa:2694;
Human Gastric intrinsic factor Protein SEQUENCE
SEQUENCE 417 AA; 45416 MW; F8B376E03A3D0F3C CRC64;
MAWFALYLLS LLWATAGTST QTQSSCSVPS AQEPLVNGIQ VLMENSVTSS
AYPNPSILIA MNLAGAYNLK AQKLLTYQLM SSDNNDLTIG QLGLTIMALT
SSCRDPGDKV SILQRQMENW APSSPNAEAS AFYGPSLAIL ALCQKNSEAT
LPIAVRFAKT LLANSSPFNV DTGAMATLAL TCMYNKIPVG SEEGYRSLFG
QVLKDIVEKI SMKIKDNGII GDIYSTGLAM QALSVTPEPS KKEWNCKKTT
DMILNEIKQG KFHNPMSIAQ ILPSLKGKTY LDVPQVTCSP DHEVQPTLPS
NPGPGPTSAS NITVIYTINN QLRGVELLFN ETINVSVKSG SVLLVVLEEA
QRKNPMFKFE TTMTSWGLVV SSINNIAENV NHKTYWQFLS GVTPLNEGVA
DYIPFNHEHI TANFTQY
UCSC uc001noi.4; human. [P27352-1];

Category

Human GIF ELISA Kit

Human intrinsic factor ELISA Kit;Human IF ELISA Kit;Human INF ELISA Kit;Human IFMH ELISA Kit;Human TCN3 ELISA Kit;Human gastric intrinsic factor ELISA Kit;Human gastric intrinsic factor (vitamin B synthesis) ELISA Kit;